Development and comparative evaluation of SARS-CoV-2 S-RBD and N based ELISA tests in various African endemic settings

被引：7

作者：

Benabdessalem, Chaouki ^{[1
,2
]}

Ben Hamouda, Wafa ^{[1
,2
]}

Marzouki, Soumaya ^{[1
]}

Faye, Rokhaya ^{[3
]}

Mbow, Adji Astou ^{[3
]}

Diouf, Babacar ^{[3
]}

Ndiaye, Oumar ^{[3
]}

Dia, Ndongo ^{[3
]}

Faye, Ousmane ^{[3
]}

Sall, Amadou A. ^{[3
]}

Diagne, Cheikh Tidiane ^{[3
]}

Amellal, Houda ^{[4
]}

Ezzikouri, Sayeh ^{[4
]}

Mioramalala, Diary Juliannie Ny ^{[5
]}

Randrianarisaona, Fanirisoa ^{[5
]}

Trabelsi, Khaled ^{[2
,6
]}

Boumaiza, Mohamed ^{[2
,6
]}

Ben Hamouda, Sonia ^{[2
]}

Ouni, Rym ^{[2
]}

Bchiri, Soumaya ^{[2
]}

Chaaban, Amani ^{[2
,6
]}

Gdoura, Mariem ^{[7
]}

Gorgi, Yousr ^{[2
,8
]}

Sfar, Imen ^{[2
,8
]}

Yalaoui, Sadok ^{[2
,9
]}

Ben Khelil, Jalila ^{[2
,10
]}

Hamzaoui, Agnes ^{[2
,11
]}

Abdallah, Meya ^{[2
,12
]}

Cherif, Yosra ^{[2
,12
]}

Petres, Stephane ^{[13
]}

Mok, Chris Ka Pun ^{[14
,15
]}

Escrioum, Nicolas ^{[13
]}

Quesney, Sebastien ^{[13
]}

Dellagi, Koussay ^{[13
,16
]}

Schoenhals, Matthieu ^{[5
]}

Sarih, M'hammed ^{[4
]}

Vigan-Womas, Ines ^{[3
]}

Bettaieb, Jihene ^{[1
,2
]}

Rourou, Samia ^{[2
,6
]}

Barbouche, Mohamed Ridha ^{[1
,2
,17
]}

Ben Ahmed, Melika ^{[1
,2
]}

机构：

[1] Inst Pasteur Tunis, Lab Transmiss Control & Immunobiol Infect, Tunis, Tunisia

[2] Univ Tunis Manar, Tunis, Tunisia

[3] Inst Pasteur, Dakar, Senegal

[4] Inst Pasteur Maroc, Dept Parasitol & Vectorial Dis, Casablanca, Morocco

[5] Inst Pasteur Madagascar, Immunol Infect Dis, Antananarivo, Madagascar

[6] Inst Pasteur Tunis, Lab Mol Microbiol Vaccinol & Biotechnol Dev, Tunis, Tunisia

[7] Inst Pasteur Tunis, Lab Clin Virol, Tunis, Tunisia

[8] Charles Nicolle Hosp, Lab Immunol, Tunis, Tunisia

[9] Abderrahmen Mami Univ Hosp, Lab Immunol, Ariana, Tunisia

[10] Abderrahmen Mami Univ Hosp, Intens Care Unit, Life Sci Dept, Biotechnol Programme, Ariana, Tunisia

[11] Abderrahmen Mami Univ Hosp, Pneumol Dept, Ariana, Tunisia

[12] Yasminette Hosp, Dept internal Med, Ben Arous, Tunisia

[13] Inst Pasteur Paris, Paris, France

[14] Univ Hong Kong, Sch Publ Hlth, HKU Pasteur Res Pole, Hong Kong, Peoples R China

[15] Chinese Univ Hong Kong, Jockey Club Sch Publ Hlth & Primary Care, Hong Kong, Peoples R China

[16] Inst Pasteur, Pasteur Network, Paris, France

[17] Arabian Gulf Univ, Coll Med & Med Sci, Dept Microbiol Immunol & Infect Dis, Manama, Bahrain

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2023年 / 105卷 / 04期

关键词：

ELISA; Nucleoprotein N; S-RBD; COVID-19; Multicentric validation; Endemic African settings;

D O I：

10.1016/j.diagmicrobio.2023.115903

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Management of the COVID-19 pandemic relies on molecular diagnostic methods supported by serological tools. Herein, we developed S-RBD-and N-based ELISA assays useful for infection rate surveillance as well as the follow-up of acquired protective immunity against SARS-CoV-2. ELISA assays were optimized using COVID-19 Tunisian patients' sera and prepandemic controls. Assays were further validated in 3 African coun-tries with variable endemic settings. The receiver operating curve was used to evaluate the assay performances. The N-and S-RBD-based ELISA assays performances, in Tunisia, were very high (AUC: 0.966 and 0.98, respectively, p < 0.0001). Cross-validation analysis showed similar performances in different settings. Cross-reactivity, with malaria infection, against viral antigens, was noticed. In head-to-head comparisons with dif-ferent commercial assays, the developed assays showed high agreement. This study demonstrates, the added value of the developed serological assays in low-income countries, particularly in ethnically diverse popula-tions with variable exposure to local endemic infectious diseases.(c) 2023 Elsevier Inc. All rights reserved.

引用

页数：7

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