Development and comparative evaluation of SARS-CoV-2 S-RBD and N based ELISA tests in various African endemic settings

被引：7

作者：

Benabdessalem, Chaouki ^{[1
,2
]}

Ben Hamouda, Wafa ^{[1
,2
]}

Marzouki, Soumaya ^{[1
]}

Faye, Rokhaya ^{[3
]}

Mbow, Adji Astou ^{[3
]}

Diouf, Babacar ^{[3
]}

Ndiaye, Oumar ^{[3
]}

Dia, Ndongo ^{[3
]}

Faye, Ousmane ^{[3
]}

Sall, Amadou A. ^{[3
]}

Diagne, Cheikh Tidiane ^{[3
]}

Amellal, Houda ^{[4
]}

Ezzikouri, Sayeh ^{[4
]}

Mioramalala, Diary Juliannie Ny ^{[5
]}

Randrianarisaona, Fanirisoa ^{[5
]}

Trabelsi, Khaled ^{[2
,6
]}

Boumaiza, Mohamed ^{[2
,6
]}

Ben Hamouda, Sonia ^{[2
]}

Ouni, Rym ^{[2
]}

Bchiri, Soumaya ^{[2
]}

Chaaban, Amani ^{[2
,6
]}

Gdoura, Mariem ^{[7
]}

Gorgi, Yousr ^{[2
,8
]}

Sfar, Imen ^{[2
,8
]}

Yalaoui, Sadok ^{[2
,9
]}

Ben Khelil, Jalila ^{[2
,10
]}

Hamzaoui, Agnes ^{[2
,11
]}

Abdallah, Meya ^{[2
,12
]}

Cherif, Yosra ^{[2
,12
]}

Petres, Stephane ^{[13
]}

Mok, Chris Ka Pun ^{[14
,15
]}

Escrioum, Nicolas ^{[13
]}

Quesney, Sebastien ^{[13
]}

Dellagi, Koussay ^{[13
,16
]}

Schoenhals, Matthieu ^{[5
]}

Sarih, M'hammed ^{[4
]}

Vigan-Womas, Ines ^{[3
]}

Bettaieb, Jihene ^{[1
,2
]}

Rourou, Samia ^{[2
,6
]}

Barbouche, Mohamed Ridha ^{[1
,2
,17
]}

Ben Ahmed, Melika ^{[1
,2
]}

机构：

[1] Inst Pasteur Tunis, Lab Transmiss Control & Immunobiol Infect, Tunis, Tunisia

[2] Univ Tunis Manar, Tunis, Tunisia

[3] Inst Pasteur, Dakar, Senegal

[4] Inst Pasteur Maroc, Dept Parasitol & Vectorial Dis, Casablanca, Morocco

[5] Inst Pasteur Madagascar, Immunol Infect Dis, Antananarivo, Madagascar

[6] Inst Pasteur Tunis, Lab Mol Microbiol Vaccinol & Biotechnol Dev, Tunis, Tunisia

[7] Inst Pasteur Tunis, Lab Clin Virol, Tunis, Tunisia

[8] Charles Nicolle Hosp, Lab Immunol, Tunis, Tunisia

[9] Abderrahmen Mami Univ Hosp, Lab Immunol, Ariana, Tunisia

[10] Abderrahmen Mami Univ Hosp, Intens Care Unit, Life Sci Dept, Biotechnol Programme, Ariana, Tunisia

[11] Abderrahmen Mami Univ Hosp, Pneumol Dept, Ariana, Tunisia

[12] Yasminette Hosp, Dept internal Med, Ben Arous, Tunisia

[13] Inst Pasteur Paris, Paris, France

[14] Univ Hong Kong, Sch Publ Hlth, HKU Pasteur Res Pole, Hong Kong, Peoples R China

[15] Chinese Univ Hong Kong, Jockey Club Sch Publ Hlth & Primary Care, Hong Kong, Peoples R China

[16] Inst Pasteur, Pasteur Network, Paris, France

[17] Arabian Gulf Univ, Coll Med & Med Sci, Dept Microbiol Immunol & Infect Dis, Manama, Bahrain

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2023年 / 105卷 / 04期

关键词：

ELISA; Nucleoprotein N; S-RBD; COVID-19; Multicentric validation; Endemic African settings;

D O I：

10.1016/j.diagmicrobio.2023.115903

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Management of the COVID-19 pandemic relies on molecular diagnostic methods supported by serological tools. Herein, we developed S-RBD-and N-based ELISA assays useful for infection rate surveillance as well as the follow-up of acquired protective immunity against SARS-CoV-2. ELISA assays were optimized using COVID-19 Tunisian patients' sera and prepandemic controls. Assays were further validated in 3 African coun-tries with variable endemic settings. The receiver operating curve was used to evaluate the assay performances. The N-and S-RBD-based ELISA assays performances, in Tunisia, were very high (AUC: 0.966 and 0.98, respectively, p < 0.0001). Cross-validation analysis showed similar performances in different settings. Cross-reactivity, with malaria infection, against viral antigens, was noticed. In head-to-head comparisons with dif-ferent commercial assays, the developed assays showed high agreement. This study demonstrates, the added value of the developed serological assays in low-income countries, particularly in ethnically diverse popula-tions with variable exposure to local endemic infectious diseases.(c) 2023 Elsevier Inc. All rights reserved.

引用

页数：7

共 29 条

[1] SARS-CoV-2 S1 and N-based serological assays reveal rapid seroconversion and induction of specific antibody response in COVID-19 patients
Algaissi, Abdullah
Alfaleh, Mohamed A.
Hala, Sharif
Abujamel, Turki S.
Alamri, Sawsan S.
Almahboub, Sarah A.
Alluhaybi, Khalid A.
Hobani, Haya, I
Alsulaiman, Reem M.
AlHarbi, Rahaf H.
ElAssouli, M-Z Aki
Alhabbab, Rowa Y.
AlSaieedi, Ahdab A.
Abdulaal, Wesam H.
Al-Somali, Afrah A.
Alofi, Fadwa S.
Khogeer, Asim A.
Alkayyal, Almohanad A.
Mahmoud, Ahmad Bakur
Almontashiri, Naif A. M.
Pain, Arnab
Hashem, Anwar M.
[J]. SCIENTIFIC REPORTS, 2020, 10 (01)
[2] A comprehensive, longitudinal analysis of humoral responses specific to four recombinant antigens of SARS-CoV-2 in severe and non-severe COVID-19 patients
Chen, Yuxin
Tong, Xin
Li, Yang
Gu, Bin
Yan, Jiawei
Liu, Yong
Shen, Han
Huang, Rui
Wu, Chao
[J]. PLOS PATHOGENS, 2020, 16 (09)
[3] Antibodies against the SARS-CoV-2 S1-RBD cross-react with dengue virus and hinder dengue pathogenesis
Cheng, Yi-Ling
Chao, Chiao-Hsuan
Lai, Yen-Chung
Hsieh, Kun-Han
Wang, Jen-Ren
Wan, Shu-Wen
Huang, Hong-Jyun
Chuang, Yung-Chun
Chuang, Woei-Jer
Yeh, Trai-Ming
[J]. FRONTIERS IN IMMUNOLOGY, 2022, 13
[4] COVID-19 in Tunisia (North Africa): Seroprevalence of SARS-CoV-2 in the General Population of the Capital City Tunis
Cherif, Ines
Kharroubi, Ghassen
Chaabane, Sana
Yazidi, Rihab
Dellagi, Mongi
Snoussi, Mohamed Ali
Salem, Sadok
Marzouki, Soumaya
Rebai, Wafa Kammoun
Rourou, Samia
Dellagi, Koussay
Barbouche, Mohamed Ridha
Benabdessalem, Chaouki
Ben Ahmed, Melika
Bettaieb, Jihene
[J]. DIAGNOSTICS, 2022, 12 (04)
[5] Rapid High-Yield Production of Functional SARS-CoV-2 Receptor Binding Domain by Viral and Non-Viral Transient Expression for Pre-Clinical Evaluation
Farnos, Omar
Venereo-Sanchez, Alina
Xu, Xingge
Chan, Cindy
Dash, Shantoshini
Chaabane, Hanan
Sauvageau, Janelle
Brahimi, Fouad
Saragovi, Uri
Leclerc, Denis
Kamen, Amine A.
[J]. VACCINES, 2020, 8 (04) : 1 - 20
[6] Fehr AR, 2015, METHODS MOL BIOL, V1282, P1, DOI 10.1007/978-1-4939-2438-7_1
[7] SARS-CoV-2 Cross-Reactivity in Prepandemic Serum from Rural Malaria-Infected Persons, Cambodia
Grassia, Jillian T.
Markwalter, Christine F.
O'Meara, Wendy P.
Taylor, Steve M.
Obala, Andrew A.
[J]. EMERGING INFECTIOUS DISEASES, 2022, 28 (05) : 1080 - 1081
[8] A comparison of four serological assays for detecting anti-SARS-CoV-2 antibodies in human serum samples from different populations
Grzelak, Ludivine
Temmam, Sarah
Planchais, Cyril
Demeret, Caroline
Tondeur, Laura
Huon, Christele
Guivel-Benhassine, Florence
Staropoli, Isabelle
Chazal, Maxime
Dufloo, Jeremy
Planas, Delphine
Buchrieser, Julian
Rajah, Maaran Michael
Robinot, Remy
Porrot, Francoise
Albert, Melanie
Chen, Kuang-Yu
Crescenzo-Chaigne, Bernadette
Donati, Flora
Anna, Francois
Souque, Philippe
Gransagne, Marion
Bellalou, Jacques
Nowakowski, Mireille
Backovic, Marija
Bouadma, Lila
Le Fevre, Lucie
Le Hingrat, Quentin
Descamps, Diane
Pourbaix, Annabelle
Laouenan, Cedric
Ghosn, Jade
Yazdanpanah, Yazdan
Besombes, Camille
Jolly, Nathalie
Pellerin-Fernandes, Sandrine
Cheny, Olivia
Ungeheuer, Marie-Noelle
Mellon, Guillaume
Morel, Pascal
Rolland, Simon
Rey, Felix A.
Behillil, Sylvie
Enouf, Vincent
Lemaitre, Audrey
Creach, Marie-Aude
Petres, Stephane
Escriou, Nicolas
Charneau, Pierre
Fontanet, Arnaud
[J]. SCIENCE TRANSLATIONAL MEDICINE, 2020, 12 (559)
[9] Characteristics of SARS-CoV-2 and COVID-19
Hu, Ben
Guo, Hua
Zhou, Peng
Shi, Zheng-Li
[J]. NATURE REVIEWS MICROBIOLOGY, 2021, 19 (03) : 141 - 154
[10] Characteristics of Anti-SARS-CoV-2 Antibodies in Recovered COVID-19 Subjects
Huynh, Angela
Arnold, Donald M.
Smith, James W.
Moore, Jane C.
Zhang, Ali
Chagla, Zain
Harvey, Bart J.
Stacey, Hannah D.
Ang, Jann C.
Clare, Rumi
Ivetic, Nikola
Chetty, Vasudhevan T.
Bowdish, Dawn M. E.
Miller, Matthew S.
Kelton, John G.
Nazy, Ishac
[J]. VIRUSES-BASEL, 2021, 13 (04):

← 1 2 3 →