New approach for in-house production of [68Ga]Ga-NOTA-UBI(29-41) using a titanium dioxide column-based generator

Efforts have been dedicated to distinguishing between infections and sterile inflammations. This study presents a novel approach for in-house synthesis of [Ga-68]Ga-NOTA-UBI(29-41) employing a titanium dioxide column-based Ga-68-generator. The optimized method yielded the radiopeptide with high radiochemical purity (> 99%) and stability (up to 90 min). [Ga-68]Ga-NOTA-UBI(29-41) demonstrates hydrophilic characteristics (Log P = - 3.57 +/- 0.20) and a binding to serum proteins of similar to 60%. The affinity of [Ga-68]Ga-NOTA-UBI(29-41) to bacteria was directly proportional to the increasing quantity of bacterial cells. Consequently, this method generates [Ga-68]Ga-NOTA-UBI(29-41) with remarkable physicochemical characteristics and high binding affinity to bacterial cells, rendering it suitable for clinical applications.