Characterization of atypical pathogenic Aeromonas salmonicida isolated from a diseased Siberian sturgeon (Acipenser baerii)

被引：5

作者：

Bakiyev, Serik ^{[1
,2
]}

Smekenov, Izat ^{[1
,2
]}

Zharkova, Irina ^{[3
]}

Kobegenova, Saidina ^{[3
]}

Sergaliyev, Nurlan ^{[4
]}

Absatirov, Gaisa ^{[5
]}

Bissenbaev, Amangeldy ^{[1
,2
,6
]}

机构：

[1] Al Farabi Kazakh Natl Univ, Fac Biol & Biotechnol, Dept Mol Biol & Genet, Alma Ata 050040, Kazakhstan

[2] Al Farabi Kazakh Natl Univ, Sci Res Inst Biol & Biotechnol Problems, Alma Ata 050040, Kazakhstan

[3] Al Farabi Kazakh Natl Univ, Fac Biol & Biotechnol, Dept Biodivers & Bioresources, Alma Ata 050040, Kazakhstan

[4] Makhambet Utemisov West Kazakhstan Univ, Uralsk 090000, Kazakhstan

[5] West Kazakhstan Innovat & Technol Univ, Uralsk 090000, Kazakhstan

[6] Al Farabi Kazakh Natl Univ, Fac Biol & Biotechnol, Dept Mol Biol & Genet, Alma Ata 050040, Kazakhstan

来源：

HELIYON | 2023年 / 9卷 / 07期

关键词：

Acipenser baerii; Aeromonas salmonicida; Oreochromis mossambicus; Pathogenicity; Virulence gene; Antibiotic resistance; VIRULENCE FACTORS; CHOLESTEROL ACYLTRANSFERASE; PHENOTYPIC CHARACTERISTICS; FRESH-WATER; A-LAYER; HYDROPHILA; INFECTION; GENES; FURUNCULOSIS; GYRB;

D O I：

10.1016/j.heliyon.2023.e17775

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Acipenser baerii (Siberian sturgeon) is native to Kazakhstan and is currently endangered and is listed within the first class of protected animals. Sturgeon aquaculture is becoming an important tool for the recovery of this endangered species. Nonetheless, diseases involving typical symptoms of skin ulceration and systemic bacterial hemorrhagic septicemia have occurred in cultured A. baerii on a fish farm located in Western Kazakhstan. In this study, an infectious strain of bacteria isolated from an ulcer of diseased A. baerii was identified as Aeromonas salmonicida (strain AB001). This identification involved analyses of 16S rRNA, gyrB, rpoD, and flaA genes' sequences. Even though strain AB001 belongs to A. salmonicida, it exhibited noticeable mobility and growth at temperatures of & GE;37 degrees C. Profiling of virulence genes uncovered the presence of seven such genes related to pathogenicity. Antibiotic sensitivity testing showed that the strain is sensitive to aminoglycosides, amphenicols, nitrofurans, quinolones, and tetracyclines. Half-lethal doses (LD50) of strain AB001 for Oreochromis mossambicus and A. baerii were determined: respectively 1.7 x 108 and 7.2 x 107 colony-forming units per mL. The experimentally induced infection revealed that strain AB001 causes considerable histological lesions in O. mossambicus, including tissue degeneration, necrosis, and hemorrhages of varied severity.

引用

页数：17