To deal with the problems of high cost in super long glass manufacturing, which restricts their applications and development in glass structures, spliced glass beams (SGB) with stainless steel bolts connections was developed. The bending deformation of the SGB with stainless steel bolts have not been elucidated because it is difficult to achieve full beam equal stiffness in the splicing part. In view of this, the conjugate beam method was utilized to calculate the bending deformation of the SGB with stainless steel bolts. The bending deformation of the SGB with stainless steel bolts was investigated by the bending performance test, while the location of abrupt stiffness change and the actual correction coefficient were determined. It is verified by finite element analysis, on the basis of which the influence law of the height-to-span ratio and the height-to-thickness ratio of glass and stainless steel plate on the ultimate load and maximum deflection of SGB with stainless steel bolts were studied. The results confirmed that SGB composed of as few stainless steel bolts as possible can fully utilize the bending capacity of glass. This study provides a simplified calculation method for the engineering design of SGB, and opens up an idea for the design of glass structures.