SARS-CoV-2 Spike Protein and Neutralizing Anti-Spike Protein Antibodies Modulate Blood Platelet Function

被引：2
作者：
Luzak, Boguslawa [1 ]
Rozalski, Marcin [1 ]
Przygodzki, Tomasz [1 ]
Boncler, Magdalena [1 ]
Wojkowska, Dagmara [1 ]
Kosmalski, Marcin [2 ]
Watala, Cezary [1 ]
机构：
[1] Med Univ Lodz, Dept Hemostasis & Hemostat Disorders, PL-92215 Lodz, Poland
[2] Med Univ Lodz, Chair Internal Dis 1, Dept Clin Pharmacol, PL-90153 Lodz, Poland
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2023年 / 24卷 / 06期
关键词：
SARS-CoV-2 spike protein; COVID-19; anti-SARS-CoV-2 IgG antibodies; blood platelets; platelet reactivity; platelet adhesion; platelet activation; COVID-19;
D O I：
10.3390/ijms24065312
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Several studies report elevated blood platelet activation and altered platelet count in COVID-19 patients, but the role of the SARS-CoV-2 spike protein in this process remains intriguing. Additionally, there is no data that anti-SARS-CoV-2 neutralizing antibodies (nAb) may attenuate spike protein activity toward blood platelets. Our results indicate that under in vitro conditions, the spike protein increased the collagen-stimulated aggregation of isolated platelets and induced the binding of vWF to platelets in ristocetin-treated blood. The spike protein also significantly reduced collagen- or ADP-induced aggregation or decreased GPIIbIIIa (fibrinogen receptor) activation in whole blood, depending on the presence of the anti-spike protein nAb. Our findings suggest that studies on platelet activation/reactivity in COVID-19 patients or in donors vaccinated with anti-SARS-CoV-2 and/or previously-infected COVID-19 should be supported by measurements of spike protein and IgG anti-spike protein antibody concentrations in blood.
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