Mechanism of Baihu Renshen decoction on T2DM rats based on mitochondrial autophagy mediated by PINK1/Parkin

Background: This study will be aimed at investigating the effects of Baihu Renshen decoction (BHRS) on type 2 diabetes rats and on macromolecular enzyme 1 (PINK1)/E3 ubiquitin protein ligase (Parkin) pathway. Methods: The experiment was divided into four groups: control group, model group, metformin group and BHRS low-dose group and high-dose group. Forty male rats were selected as samples and randomly assigned to at least one test group. Finally, there are 18 rats in each group. Except for the control group, the rats within the different teams got a high-fat diet associate in nursing an intraperitoneal injection of streptozotocin to make a type 2 diabetes mellitus (T2DM) rat model. The organic chemistry and inflammatory indexes of rats in every cluster were analyzed and compared once four weeks of intragastric administration of comparable reagents to review the therapeutic impact of BHRS on T2DM. In addition, we determined the pathological changes of ductal gland tissue of T2DM rats after treatment, and compared the expression of mitochondrial phagocytosis related proteins in ductal gland tissue of rats in each group. Results: FBG, LDL-C, TC, TG, MDA, IL-1, IL-6, TNF-, and mitophagy-related proteins COXIV, P62, VDAC1, and TOM20 were elevated in the model group compared to the control group, while HDL-C, SOD, GSH-Px, and mitophagy-related proteins PINK1, Parkin, and LC3II/I were decreased (P < 0.05 or P < 0.01). The expressions of FBG, TC, TG, LDL-C, MDA, IL-1, IL-6, TNF-, and mitophagy-related proteins COXIV, P62, VDAC1, and TOM20 were lowered in the BHRS group, while the expressions of HOMA-, HDL-C, SOD, GSH-Px, and mitophagy-related proteins PINK1, Parkin, and LC3II/I were (P < 0.05 or P < 0.01). After therapy with BHRS, hematoxylin-eosin staining showed that the intensity of pancreatic acinar staining increased, and islet cells became clear boundaries that were, regularly arranged, and with reduced vacuoles reduced. Conclusion: BHRS has a clear therapeutic effect on T2DM, which may be achieved by regulating mitochondrial autophagy through the PINK1/Parkin pathway.