The Energies of Activation and Deactivation of 2,4-Dichlorophenol Degradation by Horseradish Peroxidase Immobilized on the Modified Nanofibrous Membrane

Featured Application The obtained results can be used in the design and modeling of industrial degradation of 2,4-dichlorophenol when using an enzyme membrane with horseradish peroxidase.Abstract Chlorophenol compounds pose a health risk to many organisms due to their toxicity. The present paper presents the estimation of the activation and deactivation energies and the optimum temperatures of 2,4-dichlorophenol degradation by horseradish peroxidase (HRP). The activities of horseradish peroxidase depending on temperature were analyzed. In a mathematical model, describing 2,4-dichlorophenol degradation by HRP was assumed that both the 2,4-dichlorophenol degradation and the deactivation of HRP were first-order reactions by the enzyme concentration. The parameters of the optimum temperatures Topt, the activation energies Er, and the deactivation energies Ed in the process of 2,4-dichlorophenol degradation by HRP immobilized on a modified nanofibrous membrane were determined kd and t1/2 were determined for HRP immobilized at temperatures in the range of 25 degrees C to 75 degrees C. Likewise, thermodynamic parameters such as the change in the enthalpy increment H#, change in entropy increment S#, the change in Gibbs free energy increment G# for native HPR and the change in the enthalpy increment Hd#, change in entropy increment Sd#, and the change in Gibbs free energy increment Gd# for deactivated HRP were determined at 25 degrees C.