Haplotype-resolved chromosomal-level assembly of wasabi (Eutrema japonicum) genome

被引：3
作者：
Tanaka, Hiroyuki [1 ]
Hori, Tatsuki [1 ]
Yamamoto, Shohei [2 ]
Toyoda, Atsushi [3 ]
Yano, Kentaro [4 ]
Yamane, Kyoko [2 ]
Itoh, Takehiko [1 ]
机构：
[1] Tokyo Inst Technol, Sch Life Sci & Technol, Meguro-ku, Tokyo 1528550, Japan
[2] Gifu Univ, Fac Appl Biol Sci, 1-1 Yanagido, Gifu, Gifu 5011193, Japan
[3] Natl Inst Genet, Comparat Genom Lab, Mishima, Shizuoka 4118540, Japan
[4] Tokyo Metropolitan Univ, Dept Biol Sci, Tokyo 1920397, Japan
来源：
SCIENTIFIC DATA | 2023年 / 10卷 / 01期
关键词：
PHYLOGENETIC ANALYSIS; PROVIDES; SYSTEM; MAFFT;
D O I：
10.1038/s41597-023-02356-z
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In Japan, wasabi (Eutrema japonicum) is an important traditional condiment, and is recognized as an endemic species. In the present study, we generated a chromosome-level and haplotype-resolved reference genome for E. japonicum using PacBio CLR (continuous long reads), Illumina, and Hi-C sequencing data. The genome consists of 28 chromosomes that contain 1,512.1 Mb of sequence data, with a scaffold N50 length of 55.67 Mb. We also reported the subgenome and haplotype assignment of the 28 chromosomes by read-mapping and phylogenic analysis. Three validation methods (Benchmarking Universal Single-Copy Orthologs, Merqury, and Inspector) indicated that our obtained genome sequences were a high-quality and high-completeness genome assembly. Comparison of genome assemblies from previously published genomes showed that our obtained genome was of higher quality. Therefore, our genome will serve as a valuable genetic resource for both chemical ecology and evolution research of the genera Eutrema and Brassicaceae, as well as for wasabi breeding.
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页数：12
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