A portable 3D-printed pretreatment device combined with graded lateral flow assay for detection of S. aureus

被引：16

作者：

Chen, Yang ^{[1
]}

Zhang, Min ^{[1
]}

Wang, Xin ^{[1
]}

Majid, Zainabu ^{[1
]}

Huang, Kunlun ^{[1
,2
]}

Xu, Wentao ^{[1
,2
]}

Luo, Yunbo ^{[1
,2
]}

Nan, Cheng ^{[1
,2
]}

机构：

[1] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing Lab Food Qual & Safety, Beijing 100083, Peoples R China

[2] Minist Agr, Key Lab Safety Assessment Genet Modified Organism, Beijing 100083, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2023年 / 383卷

关键词：

Magnetic separation; Lateral flow assay; Staphylococcus aureus; Pd@Pt nanoparticles; 3D-printed device; STAPHYLOCOCCUS-AUREUS; NANOZYME; FOOD;

D O I：

10.1016/j.snb.2023.133601

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

There is a need for facile yet reliable bacterial pretreatment and signal-graded strategies for current pathogens' point-of-care testing (POCT). Herein, we present a multifunction and portable 3D-printed magnetic separation device with graded lateral flow assay (LFA) for semi-automated pretreatment and rapid detection of Staphylo-coccus aureus (S. aureus) in food. The 3D-printed pretreatment device allows purification and enrichment of the palladium@platinum nanoparticles (Pd@Pt NPs)-antibody -S. aureus complex. The recovery rate was 81.7-96.1% in actual food samples. Pd@Pt NPs-mediated signal amplification immunoassay achieved graded detection of S. aureus, reducing the detection limit from 103 CFU/mL to 102 CFU/mL, corresponding to the highest safety limit value (M) and acceptable level limit value (m) of S. aureus in many countries and organizations, respec-tively. This study paves the way for cost-effective and sensitive POCT with multiplexing potential in applications ranging from pretreatment of samples in the complex matrix as well as foodborne pathogens graded and rapid analysis.

引用

页数：8

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