Characterization of a novel bifunctional enzyme from buffalo rumen metagenome and its effect on in vitro ruminal fermentation and microbial community composition
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作者:
Meng, Zhenxiang
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Nanjing Agr Univ, Natl Ctr Int Res Anim Gut Nutr, Lab Gastrointestinal Microbiol, Nanjing 210095, Peoples R ChinaNanjing Agr Univ, Natl Ctr Int Res Anim Gut Nutr, Lab Gastrointestinal Microbiol, Nanjing 210095, Peoples R China
Meng, Zhenxiang
[1
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Ma, Jing
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Nanjing Agr Univ, Natl Ctr Int Res Anim Gut Nutr, Lab Gastrointestinal Microbiol, Nanjing 210095, Peoples R ChinaNanjing Agr Univ, Natl Ctr Int Res Anim Gut Nutr, Lab Gastrointestinal Microbiol, Nanjing 210095, Peoples R China
Ma, Jing
[1
]
Sun, Zhanying
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Nanjing Agr Univ, Natl Ctr Int Res Anim Gut Nutr, Lab Gastrointestinal Microbiol, Nanjing 210095, Peoples R ChinaNanjing Agr Univ, Natl Ctr Int Res Anim Gut Nutr, Lab Gastrointestinal Microbiol, Nanjing 210095, Peoples R China
Sun, Zhanying
[1
]
Yang, Chengjian
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Chinese Acad Agr, Buffalo Res Inst, Nanning 530000, Peoples R ChinaNanjing Agr Univ, Natl Ctr Int Res Anim Gut Nutr, Lab Gastrointestinal Microbiol, Nanjing 210095, Peoples R China
Yang, Chengjian
[3
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Leng, Jing
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Yunnan Agr Univ, Key Lab Anim Nutr & Feed Sci Yunnan Prov, Kunming 650000, Peoples R ChinaNanjing Agr Univ, Natl Ctr Int Res Anim Gut Nutr, Lab Gastrointestinal Microbiol, Nanjing 210095, Peoples R China
Leng, Jing
[4
]
Zhu, Weiyun
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Nanjing Agr Univ, Natl Ctr Int Res Anim Gut Nutr, Lab Gastrointestinal Microbiol, Nanjing 210095, Peoples R ChinaNanjing Agr Univ, Natl Ctr Int Res Anim Gut Nutr, Lab Gastrointestinal Microbiol, Nanjing 210095, Peoples R China
Zhu, Weiyun
[1
]
Cheng, Yanfen
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Nanjing Agr Univ, Natl Ctr Int Res Anim Gut Nutr, Lab Gastrointestinal Microbiol, Nanjing 210095, Peoples R China
Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Ctr Grassland Microbiome, State Key Lab Grassland Agroecosystems, Lanzhou 730000, Peoples R ChinaNanjing Agr Univ, Natl Ctr Int Res Anim Gut Nutr, Lab Gastrointestinal Microbiol, Nanjing 210095, Peoples R China
Cheng, Yanfen
[1
,2
]
机构:
[1] Nanjing Agr Univ, Natl Ctr Int Res Anim Gut Nutr, Lab Gastrointestinal Microbiol, Nanjing 210095, Peoples R China
[2] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Ctr Grassland Microbiome, State Key Lab Grassland Agroecosystems, Lanzhou 730000, Peoples R China
[3] Chinese Acad Agr, Buffalo Res Inst, Nanning 530000, Peoples R China
[4] Yunnan Agr Univ, Key Lab Anim Nutr & Feed Sci Yunnan Prov, Kunming 650000, Peoples R China
To efficiently use lignocellulosic materials in ruminants, it is crucial to explore effective enzymes, especially bifunctional enzymes. In this study, a novel stable bifunctional cellulase-xylanase protein from buffalo rumen metagenome was expressed and characterized, CelXyn2. The enzyme displayed optimal activity at pH 6.0 and 45 degrees C. The residual endoglucanase and xylanase activities were 90.6% and 86.4% after a 60-min pre-incubation at 55 degrees C. Hydrolysis of rice straw, wheat straw, sheepgrass and sugar beet pulp by CelXyn2 showed its ability to degrade both cellulose and hemicellulose polymers. Treatment with CelXyn2 improved the hydrolysis of agricultural residues with an evident increase in production of total gas, lactate and volatile fatty acids. The results of 16S rRNA and real- time PCR showed that the effect on in vitro ruminal microbial community depended on fermenta- tion substrates. This study demonstrated that CelXyn2 could strengthen lignocellulose hydrolysis and in vitro ruminal fermentation. These characteristics of CelXyn2 distinguish it as a promising candidate for agricultural application.(c) 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by- nc-nd/4.0/).
机构:
Univ Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Inst Anim Nutr & Funct Plant Cpds, Vet Pl 1, A-1210 Vienna, Austria
Univ Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Res Cluster Anim Gut Hlth, A-1210 Vienna, AustriaUniv Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Inst Anim Nutr & Funct Plant Cpds, Vet Pl 1, A-1210 Vienna, Austria
Khiaosa-ard, Ratchaneewan
Metzler-Zebeli, Barbara U.
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Univ Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Univ Clin Swine, Vet Pl 1, A-1210 Vienna, Austria
Univ Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Res Cluster Anim Gut Hlth, A-1210 Vienna, AustriaUniv Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Inst Anim Nutr & Funct Plant Cpds, Vet Pl 1, A-1210 Vienna, Austria
Metzler-Zebeli, Barbara U.
Klevenhusen, Fenja
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Univ Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Inst Anim Nutr & Funct Plant Cpds, Vet Pl 1, A-1210 Vienna, Austria
Univ Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Res Cluster Anim Gut Hlth, A-1210 Vienna, AustriaUniv Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Inst Anim Nutr & Funct Plant Cpds, Vet Pl 1, A-1210 Vienna, Austria
Klevenhusen, Fenja
Chizzola, Remigius
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Univ Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Inst Anim Nutr & Funct Plant Cpds, Vet Pl 1, A-1210 Vienna, AustriaUniv Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Inst Anim Nutr & Funct Plant Cpds, Vet Pl 1, A-1210 Vienna, Austria
Chizzola, Remigius
Zebeli, Qendrim
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Univ Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Inst Anim Nutr & Funct Plant Cpds, Vet Pl 1, A-1210 Vienna, Austria
Univ Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Res Cluster Anim Gut Hlth, A-1210 Vienna, AustriaUniv Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Inst Anim Nutr & Funct Plant Cpds, Vet Pl 1, A-1210 Vienna, Austria