Combined sulfuric acid and choline chloride/glycerol pretreatment for efficiently enhancing enzymatic saccharification of reed stalk

In this study, an efficient combination of pretreatment solvents involving Choline chloride/Glycerol (ChCl/Gly) and H2SO4 was firstly developed to assess the pretreatment performance and determine optimal pretreatment conditions. The results illustrated that the H2SO4-[ChCl/Gly] combination efficiently removed lignin (52.6%) and xylan (80.5%) from the pretreated reed stalk, and subsequent enzymatic hydrolysis yielded 91.1% of glucose. Furthermore, several characterizations were conducted to examine the structural and morphological changes of the reed stalk, revealing apparently enhanced accessibility (128.4 to 522.6 mg/g), reduced lignin surface area (357.9 to 229.5 m2/g), and substantial changes on biomass surface. Based on the aforementioned study, possible mechanisms for the H2SO4-[ChCl/Gly] pretreatment of reed stalks were proposed. The compre-hensive understanding of combined H2SO4-[ChCl/Gly] pretreatment system for enhancing the saccharification of the reed stalk was interpreted in this work. Overall, this novel approach could be efficiently applied to pretreat and saccharify reed stalks, empowering the biomass refining industry.