Studying paraben-induced estrogen receptor- and steroid hormone-related endocrine disruption effects via multi-level approaches

被引：21

作者：

Liang, Jiefeng ^{[1
,2
,6
]}

Liu, Qian S. ^{[1
]}

Ren, Zhihua ^{[1
,3
]}

Min, Ke ^{[1
]}

Yang, Xiaoxi ^{[1
]}

Hao, Fang ^{[4
,5
]}

Zhang, Qing ^{[1
]}

Liu, Qian ^{[1
,3
]}

Zhou, Qunfang ^{[1
,3
]}

Jiang, Guibin ^{[1
,3
]}

机构：

[1] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China

[2] Univ Chinese Acad Sci, Sino Danish Coll, Beijing 100049, Peoples R China

[3] Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China

[4] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China

[5] Jiangnan Univ, Inst Analyt Food Safety, Sch Food Sci & Technol, Wuxi 214122, Peoples R China

[6] Shandong Univ, Sch Environm Sci & Engn, Shandong Key Lab Environm Proc & Hlth, Qingdao 266237, Peoples R China

来源：

SCIENCE OF THE TOTAL ENVIRONMENT | 2023年 / 869卷

基金：

中国国家自然科学基金;

关键词：

Parabens; Estrogen receptor; Steroid hormone; Endocrine disruption effect; SYNTHETIC PHENOLIC ANTIOXIDANTS; BISPHENOL-A; IN-VITRO; DEVELOPMENTAL TOXICITY; BUTYL PARABEN; ZEBRAFISH; EXPOSURE; STEROIDOGENESIS; EXPRESSION; PROGESTERONE;

D O I：

10.1016/j.scitotenv.2023.161793

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Increasing concerns have been raised on the health risks of parabens in the regard of their widespread applications and potential endocrine disrupting activities. In this study, four typical parabens, including methyl paraben (MeP), ethyl paraben (EtP), propyl paraben (PrP), and butyl paraben (BuP) were systematically investigated for their estrogen receptor-and steroid hormone-related endocrine disruptions using multi-level approaches. Paraben exposure pro-moted the proliferation of MCF-7 cells, increased the luciferase activity in MVLN cells, and induced the vitellogenin (vtg) expression in zebrafish larvae, showing the typical estrogenic effects. The in vitro protein assays further revealed that PrP and BuP could bind with two isoforms of estrogen receptors (ERs). The estrogenic activities of parabens were predicted to be positively correlated with their chemical structure complexity by using molecular docking analysis. Furthermore, the synthesis and secretion of estradiol (E2) and testosterone (T) were significantly disturbed in H295R cells and zebrafish larvae, which could be regulated by paraben-induced transcriptional disturbance in both in vitro steroidogenesis and in vivo hypothalamic-pituitary-gonadal (HPG) axis. Parabens could disturb the endocrine system by activating the ERs and disrupting the steroid hormone synthesis and secretion, suggesting their potential del-eterious risks to the environment and human health.

引用

页数：10