Citrate and glutathione capped gold nanoparticles for electrochemical immunosensing of atrazine: Effect of conjugation chemistry

Recently, the exposure of pesticides/herbicides to the living organisms is increased especially due to agricultural malpractices and industrial processes. In particular, the exposure of pesticides/herbicides (e.g., atrazine) can impart several harsh effects on the human health. The development of efficient detection systems can be crucial in monitoring the atrazine in water and food/plant products, which can be decisive in controlling the deadly exposures of atrazine. Herein, we have developed electrochemical immunosensors for atrazine by employing monoclonal anti-atrazine antibody conjugated gold nanoparticles. Two types of gold nanoparticles (i.e., citrate and glutathione (GSH)-capped AuNPs) were used to modify gold working electrode and utilized for the development of atrazine immunosensors. The conjugation of immunoprobe on working electrode was especially designed to obtain stable and efficient sensing signals. The nanosensing immunoprobes fabricated using citrateAuNPs and GSH-AuNPs exhibited comparable responses for a wide linear working range of 50 ng/L- 30 mu g/L with limit of detection (LOD) values of 0.08 and 0.06 ng/L for atrazine, respectively.