Stimulated Raman scattering microscopy on biological cellular machinery

被引:10
作者
Huang, Jing [1 ,2 ]
Ji, Minbiao [1 ,2 ,3 ]
机构
[1] Fudan Univ, State Key Lab Surface Phys, Human Phenome Inst,Minist Educ, Acad Engn & Technol,Key Lab Micro & Nanophoton St, Shanghai 200433, Peoples R China
[2] Fudan Univ, Dept Phys, Human Phenome Inst,Minist Educ, Acad Engn & Technol,Key Lab Micro & Nanophoton St, Shanghai 200433, Peoples R China
[3] Fudan Univ, Yiwu Res Inst, Chengbei Rd, Yiwu 322000, Zhejiang, Peoples R China
基金
国家重点研发计划; 中国国家自然科学基金;
关键词
Nonlinear optical microscopy; vibrational spectral imaging; coherent Raman scattering; biological cells; IMAGING IN-VIVO; LIVE CELLS; INFRARED-SPECTROSCOPY; METABOLIC MARKER; CHEMICAL-BONDS; FLUORESCENCE; TISSUES; IDENTIFICATION; SENSITIVITY; RESOLUTION;
D O I
10.1142/S1793545822300105
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Benefiting from the developments of advanced optical microscopy techniques, the mysteries of biological functions at the cellular and subcellular levels have been continuously revealed. Stimulated Raman scattering (SRS) microscopy is a rapidly growing technique that has attracted broad attentions and become a powerful tool for biology and biomedicine, largely thanks to its chemical specificity, high sensitivity and fast image speed. This review paper introduces the principles of SRS, discusses the technical developments and implementations of SRS microscopy, then highlights and summarizes its applications on biological cellular machinery and finally shares our visions of potential breakthroughs in the future.
引用
收藏
页数:17
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