Characterization of immune populations in the tumor microenvironment of diffuse midline glioma orthotopic mouse models by flow cytometry

被引：0
作者：
Mauleon, Iker Ausejo- [1 ,2 ,3 ]
Labiano, Sara [1 ,2 ,3 ]
Alonso, Marta M. [1 ,2 ,3 ]
机构：
[1] Hlth Res Inst Navarra IdiSNA, Pamplona, Spain
[2] Univ Navarra, CIMA, Solid Tumor Program, Pamplona, Spain
[3] Clin Univ Navarra, Dept Pediat, Pamplona, Spain
来源：
STAR PROTOCOLS | 2024年 / 5卷 / 01期
基金：
欧洲研究理事会;
关键词：
Cancer; Flow Cytometry; Immunology;
D O I：
10.1016/j.xpro.2023.102803
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The immune response is a fundamental process in the treatment of solid tumors. Here, we present a protocol for implanting diffuse midline glioma cells in the brain of immune-competent mice and characterizing the different immune pop-ulations in the tumor microenvironment in a flow cytometry panel. We describe steps for processing of brain tumors, isolating the immune cells, and subsequent staining with antibodies for flow cytometry. We then detail procedures for imple-menting the gating strategy. For complete details on the use and execution of this protocol, please refer to Ausejo-Mauleon et al.1
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页数：16
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