Microbiological Non-Culture-Based Methods for Diagnosing Invasive Pulmonary Aspergillosis in ICU Patients

被引：2

作者：

Scharmann, Ulrike ^{[1
]}

Verhasselt, Hedda Luise ^{[1
]}

Kirchhoff, Lisa ^{[1
]}

Furnica, Dan-Tiberiu ^{[1
]}

Steinmann, Joerg ^{[1
,2
]}

Rath, Peter-Michael ^{[1
]}

机构：

[1] Univ Duisburg Essen, Univ Hosp Essen, Inst Med Microbiol, D-45122 Essen, Germany

[2] Paracelsus Med Univ, Inst Clin Hyg Med Microbiol & Infectiol, Klinikum Nurnberg, D-90419 Nurnberg, Germany

来源：

DIAGNOSTICS | 2023年 / 13卷 / 16期

关键词：

Aspergillus; aspergillosis; fungal infections; ICU; galactomannan; (1 -> 3)-beta-d-glucan; BRONCHOALVEOLAR LAVAGE FLUID; GALACTOMANNAN; POSACONAZOLE; FLUCONAZOLE; PERFORMANCE; PROPHYLAXIS; DISEASE; HOST; TOOL;

D O I：

10.3390/diagnostics13162718

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The diagnosis of invasive pulmonary aspergillosis (IPA) in intensive care unit (ICU) patients is crucial since most clinical signs are not specific to invasive fungal infections. To detect an IPA, different criteria should be considered. Next to host factors and radiological signs, microbiological criteria should be fulfilled. For microbiological diagnostics, different methods are available. Next to the conventional culture-based approaches like staining and culture, non-culture-based methods can increase sensitivity and improve time-to-result. Besides fungal biomarkers, like galactomannan and (1 -> 3)- beta-D-glucan as nonspecific tools, molecular-based methods can also offer detection of resistance determinants. The detection of novel biomarkers or targets is promising. In this review, we evaluate and discuss the value of non-culture-based microbiological methods (galactomannan, (1 -> 3)- beta-D-glucan, Aspergillus PCR, new biomarker/targets) for diagnosing IPA in ICU patients.

引用

页数：11

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