Comparing cadmium-induced effects on the regulation of the DNA damage response and cell cycle progression between entire rosettes and individual leaves of Arabidopsis thaliana
Cadmium (Cd) activates the DNA damage response (DDR) and inhibits the cell cycle in Arabidopsis thaliana through the transcription factor SUPPRESSOR OF GAMMA RESPONSE 1. The aim of this study was to investigate which individual leaf best reflects the Cd-induced effects on the regulation of the DDR and cell cycle progression in rosettes, enabling a more profound interpretation of the rosette data since detailed information, provided by the individual leaf responses, is lost when studying the whole rosette. Wild-type A. thaliana plants were cultivated in hydroponics and exposed to different Cd concentrations. Studied individual leaves were leaf 1 and 2, which emerged before Cd exposure, and leaf 3, which emerged upon Cd exposure. The DDR and cell cycle regulation were studied in rosettes as well as individual leaves after several days of Cd exposure. Varying concentration-dependent response patterns were observed between the entire rosette and individual leaves. Gene expression of selected DDR and cell cycle regulators showed higher similarity in their response between the rosette and the individual leaf emerged during Cd exposure than between both individual leaves. The same pattern was observed for plant growth and cell cycle-related parameters. We conclude that Cd-induced effects on the regulation of the DDR and cell cycle progression in the leaf that emerged during Cd exposure, resemble those observed in the rosette the most, which contributes to the interpretation of the rosette data in the framework of plant development and after exposure to Cd.
机构:
Barts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, EnglandBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Bustin, Stephen A.
;
Benes, Vladimir
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机构:
EMBL Heidelberg, Genom Core Facil, Heidelberg, GermanyBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Benes, Vladimir
;
Garson, Jeremy A.
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机构:
UCL, Dept Infect, Ctr Virol, London, England
UCL Hosp, NHS Fdn Trust, Dept Virol, London, EnglandBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Garson, Jeremy A.
;
Hellemans, Jan
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机构:
Ghent Univ Hosp, Ctr Med Genet, B-9000 Ghent, BelgiumBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Hellemans, Jan
;
Huggett, Jim
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机构:
UCL, Ctr Infect Dis, London, EnglandBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Huggett, Jim
;
Kubista, Mikael
论文数: 0引用数: 0
h-index: 0
机构:
TATAA Bioctr, Gothenburg, Sweden
Inst Biotechnol AS CR, Prague, Czech RepublicBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Kubista, Mikael
;
Mueller, Reinhold
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h-index: 0
机构:
Sequenom, San Diego, CA USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Mueller, Reinhold
;
Nolan, Tania
论文数: 0引用数: 0
h-index: 0
机构:
Sigma Aldrich, Haverhill, MA USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Nolan, Tania
;
Pfaffl, Michael W.
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h-index: 0
机构:
Tech Univ Munich, D-8050 Freising Weihenstephan, GermanyBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Pfaffl, Michael W.
;
Shipley, Gregory L.
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机构:
Univ Texas Houston, Hlth Sci Ctr, Dept Integrat Biol & Pharmacol, Quantitat Genom Core Lab, Houston, TX USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Shipley, Gregory L.
;
Vandesompele, Jo
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Ghent Univ Hosp, Ctr Med Genet, B-9000 Ghent, BelgiumBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Vandesompele, Jo
;
Wittwer, Carl T.
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h-index: 0
机构:
Univ Utah, Dept Pathol, Salt Lake City, UT USA
ARUP Inst Clin & Expt Pathol, Salt Lake City, UT USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
机构:
Univ Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, Brazil
Carneiro, Aline Kohn
;
Montessoro, Patricia da Fonseca
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Univ Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, Brazil
Montessoro, Patricia da Fonseca
;
Fusaro, Adriana Flores
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Univ Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, Brazil
Fusaro, Adriana Flores
;
Araujo, Bruna Gino
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Univ Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, Brazil
Araujo, Bruna Gino
;
Hemerly, Adriana Silva
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Univ Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, Brazil
机构:
Barts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, EnglandBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Bustin, Stephen A.
;
Benes, Vladimir
论文数: 0引用数: 0
h-index: 0
机构:
EMBL Heidelberg, Genom Core Facil, Heidelberg, GermanyBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Benes, Vladimir
;
Garson, Jeremy A.
论文数: 0引用数: 0
h-index: 0
机构:
UCL, Dept Infect, Ctr Virol, London, England
UCL Hosp, NHS Fdn Trust, Dept Virol, London, EnglandBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Garson, Jeremy A.
;
Hellemans, Jan
论文数: 0引用数: 0
h-index: 0
机构:
Ghent Univ Hosp, Ctr Med Genet, B-9000 Ghent, BelgiumBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Hellemans, Jan
;
Huggett, Jim
论文数: 0引用数: 0
h-index: 0
机构:
UCL, Ctr Infect Dis, London, EnglandBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Huggett, Jim
;
Kubista, Mikael
论文数: 0引用数: 0
h-index: 0
机构:
TATAA Bioctr, Gothenburg, Sweden
Inst Biotechnol AS CR, Prague, Czech RepublicBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Kubista, Mikael
;
Mueller, Reinhold
论文数: 0引用数: 0
h-index: 0
机构:
Sequenom, San Diego, CA USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Mueller, Reinhold
;
Nolan, Tania
论文数: 0引用数: 0
h-index: 0
机构:
Sigma Aldrich, Haverhill, MA USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Nolan, Tania
;
Pfaffl, Michael W.
论文数: 0引用数: 0
h-index: 0
机构:
Tech Univ Munich, D-8050 Freising Weihenstephan, GermanyBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Pfaffl, Michael W.
;
Shipley, Gregory L.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Texas Houston, Hlth Sci Ctr, Dept Integrat Biol & Pharmacol, Quantitat Genom Core Lab, Houston, TX USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Shipley, Gregory L.
;
Vandesompele, Jo
论文数: 0引用数: 0
h-index: 0
机构:
Ghent Univ Hosp, Ctr Med Genet, B-9000 Ghent, BelgiumBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Vandesompele, Jo
;
Wittwer, Carl T.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Utah, Dept Pathol, Salt Lake City, UT USA
ARUP Inst Clin & Expt Pathol, Salt Lake City, UT USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
机构:
Univ Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, Brazil
Carneiro, Aline Kohn
;
Montessoro, Patricia da Fonseca
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, Brazil
Montessoro, Patricia da Fonseca
;
Fusaro, Adriana Flores
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h-index: 0
机构:
Univ Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, Brazil
Fusaro, Adriana Flores
;
Araujo, Bruna Gino
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h-index: 0
机构:
Univ Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, Brazil
Araujo, Bruna Gino
;
Hemerly, Adriana Silva
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h-index: 0
机构:
Univ Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med, Lab Biol Mol Plantas, Cidade Univ, BR-21941902 Rio De Janeiro, Brazil