Comparing cadmium-induced effects on the regulation of the DNA damage response and cell cycle progression between entire rosettes and individual leaves of Arabidopsis thaliana

被引:2
作者
Hendrix, Sophie [1 ]
Alfano, Rossella [1 ]
Plusquin, Michelle [1 ]
Cuypers, Ann [1 ]
机构
[1] Hasselt Univ, Ctr Environm Sci, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium
关键词
Arabidopsis thaliana; Cadmium; DNA damage response; Cell cycle; Cell division; Endoreplication; Rosette; Leaves; PLANTS; ENDOREPLICATION; GLUTATHIONE; STRESS; GROWTH;
D O I
10.1016/j.plaphy.2023.108105
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Cadmium (Cd) activates the DNA damage response (DDR) and inhibits the cell cycle in Arabidopsis thaliana through the transcription factor SUPPRESSOR OF GAMMA RESPONSE 1. The aim of this study was to investigate which individual leaf best reflects the Cd-induced effects on the regulation of the DDR and cell cycle progression in rosettes, enabling a more profound interpretation of the rosette data since detailed information, provided by the individual leaf responses, is lost when studying the whole rosette. Wild-type A. thaliana plants were cultivated in hydroponics and exposed to different Cd concentrations. Studied individual leaves were leaf 1 and 2, which emerged before Cd exposure, and leaf 3, which emerged upon Cd exposure. The DDR and cell cycle regulation were studied in rosettes as well as individual leaves after several days of Cd exposure. Varying concentration-dependent response patterns were observed between the entire rosette and individual leaves. Gene expression of selected DDR and cell cycle regulators showed higher similarity in their response between the rosette and the individual leaf emerged during Cd exposure than between both individual leaves. The same pattern was observed for plant growth and cell cycle-related parameters. We conclude that Cd-induced effects on the regulation of the DDR and cell cycle progression in the leaf that emerged during Cd exposure, resemble those observed in the rosette the most, which contributes to the interpretation of the rosette data in the framework of plant development and after exposure to Cd.
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页数:11
相关论文
共 54 条
[1]   Toxic Mechanisms of Five Heavy Metals: Mercury, Lead, Chromium, Cadmium, and Arsenic [J].
Balali-Mood, Mahdi ;
Naseri, Kobra ;
Tahergorabi, Zoya ;
Khazdair, Mohammad Reza ;
Sadeghi, Mahmood .
FRONTIERS IN PHARMACOLOGY, 2021, 12
[2]   Genome-wide analysis of gene expression profiles associated with cell cycle transitions in growing organs of Arabidopsis [J].
Beemster, GTS ;
De Veylder, L ;
Vercruysse, S ;
West, G ;
Rombaut, D ;
Van Hummelen, P ;
Galichet, A ;
Gruissem, W ;
Inzé, D ;
Vuylsteke, M .
PLANT PHYSIOLOGY, 2005, 138 (02) :734-743
[3]   Endoreplication Controls Cell Fate Maintenance [J].
Bramsiepe, Jonathan ;
Wester, Katja ;
Weinl, Christina ;
Roodbarkelari, Farshad ;
Kasili, Remmy ;
Larkin, John C. ;
Huelskamp, Martin ;
Schnittger, Arp .
PLOS GENETICS, 2010, 6 (06) :1-14
[4]   The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments [J].
Bustin, Stephen A. ;
Benes, Vladimir ;
Garson, Jeremy A. ;
Hellemans, Jan ;
Huggett, Jim ;
Kubista, Mikael ;
Mueller, Reinhold ;
Nolan, Tania ;
Pfaffl, Michael W. ;
Shipley, Gregory L. ;
Vandesompele, Jo ;
Wittwer, Carl T. .
CLINICAL CHEMISTRY, 2009, 55 (04) :611-622
[5]   Roles of MSH2 and MSH6 in cadmium-induced G2/M checkpoint arrest in Arabidopsis roots [J].
Cao, Xia ;
Wang, Hetong ;
Zhuang, Defeng ;
Zhu, He ;
Du, Yanli ;
Cheng, Zhibo ;
Cui, Weina ;
Rogers, Hilary J. ;
Zhang, Qianru ;
Jia, Chunjun ;
Yang, Yuesuo ;
Tai, Peidong ;
Xie, Futi ;
Liu, Wan .
CHEMOSPHERE, 2018, 201 :586-594
[6]   Plant CDKs-Driving the Cell Cycle through Climate Change [J].
Carneiro, Aline Kohn ;
Montessoro, Patricia da Fonseca ;
Fusaro, Adriana Flores ;
Araujo, Bruna Gino ;
Hemerly, Adriana Silva .
PLANTS-BASEL, 2021, 10 (09)
[7]   Growth of the plant cell wall [J].
Cosgrove, DJ .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2005, 6 (11) :850-861
[8]   Cell cycle arrest mediated by Cd-induced DNA damage in Arabidopsis root tips [J].
Cui, Weina ;
Wang, Hetong ;
Song, Jie ;
Cao, Xia ;
Rogers, Hilary J. ;
Francis, Dennis ;
Jia, Chunyun ;
Sun, Lizong ;
Hou, Meifang ;
Yang, Yuesuo ;
Tai, Peidong ;
Liu, Wan .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2017, 145 :569-574
[9]  
Cuypers A., 2022, Patent No. [2022052518W, 2022052518]
[10]   Cadmium-induced oxidative stress responses and acclimation in plants require fine-tuning of redox biology at subcellular level [J].
Cuypers, Ann ;
Vanbuel, Isabeau ;
Iven, Verena ;
Kunnen, Kris ;
Vandionant, Stephanie ;
Huybrechts, Michiel ;
Hendrix, Sophie .
FREE RADICAL BIOLOGY AND MEDICINE, 2023, 199 :81-96