Phosphatase-degradable nanoparticles: A game-changing approach for the delivery of antifungal proteins

被引：7

作者：

Akkus-Dagdeviren, Zeynep Burcu ^{[1
]}

Saleh, Ahmad ^{[1
,2
]}

Schoepf, Cristina ^{[3
]}

Truszkowska, Martyna ^{[1
]}

Bratschun-Khan, Doris ^{[3
]}

Furst, Andrea ^{[1
]}

Seybold, Anna ^{[5
]}

Offterdinger, Martin ^{[4
]}

Marx, Florentine ^{[3
]}

Bernkop-Schnuerch, Andreas ^{[1
]}

机构：

[1] Univ Innsbruck, Inst Pharm, Ctr Chem & Biomed, Dept Pharmaceut Technol, Innrain 80-82, A-6020 Innsbruck, Austria

[2] Univ Mandala Waluya, Dept Pharm, Kendari 93231, Southeast Sulaw, Indonesia

[3] Med Univ Innsbruck, Inst Mol Biol, Bioctr, Innrain 80-82, A-6020 Innsbruck, Austria

[4] Med Univ Innsbruck, Div Neurobiochem, Bioopt, Innrain 80-82, A-6020 Innsbruck, Austria

[5] Univ Innsbruck, Dept Zool, A-6020 Innsbruck, Austria

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 2023年 / 646卷

关键词：

Candida albicans; Antifungal protein; Polyphosphate; Phosphatase; Enzyme -responsive nanocarriers; Polymeric nanoparticles; Biofilm; CANDIDA-ALBICANS; DRUG-DELIVERY; MALACHITE GREEN; IN-VITRO; POLYPHOSPHATE; ENZYMES; SYSTEMS; SILVER; ASSAY;

D O I：

10.1016/j.jcis.2023.05.051

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Hypothesis: Polyphosphate nanoparticles as phosphatase-degradable carriers for Penicillium chrysogenum anti -fungal protein (PAF) can enhance the antifungal activity of the protein against Candida albicans biofilm. Experiments: PAF-polyphosphate (PP) nanoparticles (PAF-PP NPs) were obtained through ionic gelation. The resulting NPs were characterized in terms of their particle size, size distribution and zeta potential. Cell viability and hemolysis studies were carried out in vitro on human foreskin fibroblasts (Hs 68 cells) and human eryth-rocytes, respectively. Enzymatic degradation of NPs was investigated by monitoring release of free mono -phosphates in the presence of isolated as well as C. albicans-derived phosphatases. In parallel, shift in zeta potential of PAF-PP NPs as a response to phosphatase stimuli was determined. Diffusion of PAF and PAF-PP NPs through C. albicans biofilm matrix was analysed by fluorescence correlation spectroscopy (FCS). Antifungal synergy was evaluated on C. albicans biofilm by determining the colony forming units (CFU).

引用

页码：290 / 300

页数：11

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