Joint-GWAS, Linkage Mapping, and Transcriptome Analysis to Reveal the Genetic Basis of Plant Architecture-Related Traits in Maize

被引：3

作者：

Lu, Xuefeng ^{[1
,2
]}

Liu, Pengfei ^{[1
]}

Tu, Liang ^{[1
]}

Guo, Xiangyang ^{[1
]}

Wang, Angui ^{[1
]}

Zhu, Yunfang ^{[1
]}

Jiang, Yulin ^{[1
,2
]}

Zhang, Chunlan ^{[1
]}

Xu, Yan ^{[1
]}

Chen, Zehui ^{[1
]}

Wu, Xun ^{[1
,2
]}

机构：

[1] Guizhou Acad Agr Sci, Inst Upland Food Crops, Guiyang 550006, Peoples R China

[2] Minist Agr & Rural Affairs, Key Lab Crop Genet Resources & Germplasm Innovat K, Guiyang 550006, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2024年 / 25卷 / 05期

关键词：

maize (Zea mays L.); plant architecture; linkage mapping; QTL; candidate gene; TARGETED MUTAGENESIS; AGRONOMIC TRAITS; QTL; TOLERANCE; NUMBER; GROWTH; ALLELE;

D O I：

10.3390/ijms25052694

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plant architecture is one of the key factors affecting maize yield formation and can be divided into secondary traits, such as plant height (PH), ear height (EH), and leaf number (LN). It is a viable approach for exploiting genetic resources to improve plant density. In this study, one natural panel of 226 inbred lines and 150 family lines derived from the offspring of T32 crossed with Qi319 were genotyped by using the MaizeSNP50 chip and the genotyping by sequence (GBS) method and phenotyped under three different environments. Based on the results, a genome-wide association study (GWAS) and linkage mapping were analyzed by using the MLM and ICIM models, respectively. The results showed that 120 QTNs (quantitative trait nucleotides) and 32 QTL (quantitative trait loci) related to plant architecture were identified, including four QTL and 40 QTNs of PH, eight QTL and 41 QTNs of EH, and 20 QTL and 39 QTNs of LN. One dominant QTL, qLN7-2, was identified in the Zhangye environment. Six QTNs were commonly identified to be related to PH, EH, and LN in different environments. The candidate gene analysis revealed that Zm00001d021574 was involved in regulating plant architecture traits through the autophagy pathway, and Zm00001d044730 was predicted to interact with the male sterility-related gene ms26. These results provide abundant genetic resources for improving maize plant architecture traits by using approaches to biological breeding.

引用

页数：17