Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor

被引：4

作者：

Jaric, Stefan ^{[1
]}

Bajaj, Aabha ^{[2
]}

Vukic, Vladimir ^{[3
]}

Gadjanski, Ivana ^{[1
]}

Abdulhalim, Ibrahim ^{[2
,4
]}

Bobrinetskiy, Ivan ^{[1
]}

机构：

[1] Univ Novi Sad, BioSense Inst Res & Dev, Inst Informat Technol Biosyst, Novi Sad 21000, Serbia

[2] Ben Gurion Univ Negev, Ilse Katz Inst Nanoscale Sci & Technol, Sch Elect & Comp Engn, Dept Electroopt & Photon Engn, IL-84105 Beer Sheva, Israel

[3] Univ Novi Sad, Fac Technol Novi Sad, Bulevar Cara Lazara 1, Novi Sad 21000, Serbia

[4] Photonicsys Ltd, 54 Wahat Alsalam Neveh Shalom, IL-9976100 Ibrahim, Israel

来源：

TOXINS | 2023年 / 15卷 / 05期

基金：

欧盟地平线“2020”;

关键词：

CVD graphene; label-free biosensing; cyanotoxins; aptamer; surface plasmon resonance; SENSITIVITY ENHANCEMENT; BIOSENSORS; SENSORS; TOXINS; WATER;

D O I：

10.3390/toxins15050326

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In this work, we report a novel method for the label-free detection of cyanotoxin molecules based on a direct assay utilizing a graphene-modified surface plasmon resonance (SPR) aptasensor. Molecular dynamic simulation of the aptamer's interaction with cylindrospermopsin (CYN) reveals the strongest binding sites between C18-C26 pairs. To modify the SPR sensor, the wet transfer method of CVD monolayer graphene was used. For the first time, we report the use of graphene functionalized by an aptamer as a bioreceptor in conjunction with SPR for the detection of CYN. In a direct assay with an anti-CYN aptamer, we demonstrated a noticeable change in the optical signal in response to the concentrations far below the maximum tolerable level of 1 mu g/L and high specificity.

引用

页数：10

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