Biocompatibility Evaluation of Carbon Nanohorns in Bone Tissues

被引：2

作者：

Ueda, Katsuya ^{[1
]}

Ma, Chuang ^{[1
]}

Izumiya, Makoto ^{[1
]}

Kuroda, Chika ^{[2
]}

Ishida, Haruka ^{[3
]}

Uemura, Takeshi ^{[1
,3
,4
]}

Saito, Naoto ^{[3
]}

Aoki, Kaoru ^{[5
]}

Haniu, Hisao ^{[1
,3
,6
]}

机构：

[1] Shinshu Univ, Grad Sch Med Sci & Technol, Biomed Engn Div, 3-1-1 Asahi, Matsumoto, Nagano 3908621, Japan

[2] Yamaguchi Univ, Grad Sch Med, Dept Organ Anat & Nanomed, 1-1-1 Minami Kogushi Ube, Yamaguchi 7558505, Japan

[3] Shinshu Univ, Inst Biomed Sci, Interdisciplinary Cluster Cutting Edge Res, 3-1-1 Asahi, Matsumoto, Nagano 3908621, Japan

[4] Shinshu Univ, Res Ctr Adv Sci, Div Gene Res, 3-1-1 Asahi, Matsumoto, Nagano 3908621, Japan

[5] Shinshu Univ, Sch Hlth Sci, Phys Therapy Div, 3-1-1 Asahi, Matsumoto, Nagano 3908621, Japan

[6] Shinshu Univ, Sch Med, Dept Orthoped Surg, 3-1-1 Asahi, Matsumoto, Nagano 3908621, Japan

来源：

NANOMATERIALS | 2023年 / 13卷 / 02期

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

nanoparticles; carbon nanohorns; carbon blacks; osteoclast-like cells; osteoblast-like cells; biocompatibility; bone regeneration; drug delivery system; DIFFERENTIATION; TOXICITY; RELEASE;

D O I：

10.3390/nano13020244

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

With the advent of nanotechnology, the use of nanoparticles as drug delivery system (DDS) has attracted great interest. We aimed to apply carbon nanohorns (CNHs) as DDS in the development of new treatments for bone diseases. We evaluated the in vitro and in vivo cellular responses of CNHs in bone-related cells compared with carbon blacks (CBs), which are similar in particle size but differ in surface and structural morphologies. Although in vitro experiments revealed that both CNHs and CBs were incorporated into the lysosomes of RAW264-induced osteoclast-like cells (OCs) and MC3T3-E1 osteoblast-like cells (OBs), no severe cytotoxicity was observed. CNHs reduced the tartrate-resistant acid phosphatase activity and expression of the differentiation marker genes in OCs at noncytotoxic concentrations, whereas the alkaline phosphatase activity and differentiation of OBs increased. Under calcification of OBs, CNHs increased the number of calcified nodules and were intra- and extracellularly incorporated into calcified vesicles to form crystal nuclei. The in vivo experiments showed significant promotion of bone regeneration in the CNH group alone, with localized CNHs being found in the bone matrix and lacunae. The suppression of OCs and promotion of OBs suggested that CNHs may be effective against bone diseases and could be applied as DDS.

引用

页数：14