The interplay between the epithelial permeability barrier, cell migration and mitochondrial metabolism of growth factors and their inhibitors in a human endometrial carcinoma cell line

被引：2

作者：

Konno, Takumi ^{[1
]}

Kohno, Takayuki ^{[1
]}

Kikuchi, Shin ^{[2
]}

Kura, Arisa ^{[1
,3
]}

Saito, Kimihito ^{[3
]}

Okada, Tadahi ^{[3
]}

Shimada, Hiroshi ^{[3
]}

Yamazaki, Yuya ^{[1
]}

Sugiyama, Tomoki ^{[1
]}

Matsuura, Motoki ^{[3
]}

Ohsaki, Yuki ^{[2
]}

Saito, Tsuyoshi ^{[3
]}

Kojima, Takashi ^{[1
]}

机构：

[1] Sapporo Med Univ, Res Inst Frontier Med, Sch Med, Dept Cell Sci, South 1,West 17,Chuo Ku, Sapporo 0608556, Japan

[2] Sapporo Med Univ, Sch Med, Dept Anat, Sapporo, Japan

[3] Sapporo Med Univ, Sch Med, Dept Obstet & Gynecol, Sapporo, Japan

来源：

TISSUE BARRIERS | 2024年 / 12卷 / 04期

基金：

日本学术振兴会;

关键词：

Cell migration; endometrial carcinoma; epithelial permeability barrier; mitochondria metabolism; tight junctions; JUNCTIONS; BETA;

D O I：

10.1080/21688370.2024.2304443

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

It is known that there are abnormalities of tight junction functions, cell migration and mitochondrial metabolism in human endometriosis and endometrial carcinoma. In this study, we investigated the effects of growth factors and their inhibitors on the epithelial permeability barrier, cell migration and mitochondrial metabolism in 2D and 2.5D cultures of human endometrioid endometrial carcinoma Sawano cells. We also investigated the changes of bicellular and tricellular tight junction molecules and ciliogenesis induced by these inhibitors. The growth factors TGF-beta and EGF affected the epithelial permeability barrier, cell migration and expression of bicellular and tricellular tight junction molecules in 2D and 2.5D cultures of Sawano cells. EW-7197 (a TGF-beta receptor inhibitor), AG1478 (an EGFR inhibitor) and SP600125 (a JNK inhibitor) affected the epithelial permeability barrier, cell migration and mitochondrial metabolism and prevented the changes induced by TGF-beta and EGF in 2D and 2.5D cultures. EW-7197 and AG1478 induced ciliogenesis in 2.5D cultures. In conclusion, TGF-beta and EGF promoted the malignancy of endometrial cancer via interplay among the epithelial permeability barrier, cell migration and mitochondrial metabolism. EW-7197 and AG1478 may be useful as novel therapeutic treatments options for endometrial cancer.

引用

页数：16

共 38 条

[1] Biology of claudins [J].

Angelow, Susanne ;

Ahlstrom, Robert ;

Yu, Alan S. L. .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2008, 295 (04) :F867-F876

[2]

Arai Wataru, 2023, Oncotarget, V14, P261, DOI 10.18632/oncotarget.27728

[3] Interplay between tight junctions & adherens junctions [J].

Campbell, Hannah K. ;

Maiers, Jessica L. ;

DeMali, Kris A. .

EXPERIMENTAL CELL RESEARCH, 2017, 358 (01) :39-44

[4] Inhibition of basal p38 or JNK activity enhances epithelial barrier function through differential modulation of claudin expression [J].

Carrozzino, Fabio ;

Pugnale, Paolo ;

Feraille, Eric ;

Montesano, Roberto .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2009, 297 (03) :C775-C787

[5] Claudins in occluding junctions of humans and flies [J].

Furuse, M ;

Tsukita, S .

TRENDS IN CELL BIOLOGY, 2006, 16 (04) :181-188

[6] Tricellular junctions: how to build junctions at the TRICkiest points of epithelial cells [J].

Higashi, Tomohito ;

Miller, Ann L. .

MOLECULAR BIOLOGY OF THE CELL, 2017, 28 (15) :2023-2034

[7] Analysis of the 'angulin' proteins LSR, ILDR1 and ILDR2-tricellulin recruitment, epithelial barrier function and implication in deafness pathogenesis [J].

Higashi, Tomohito ;

Tokuda, Shinsaku ;

Kitajiri, Shin-ichiro ;

Masuda, Sayuri ;

Nakamura, Hiroki ;

Oda, Yukako ;

Furuse, Mikio .

JOURNAL OF CELL SCIENCE, 2013, 126 (04) :966-977

[8] Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells [J].

Ikenouchi, J ;

Furuse, M ;

Furuse, K ;

Sasaki, H ;

Tsukita, S ;

Tsukita, S .

JOURNAL OF CELL BIOLOGY, 2005, 171 (06) :939-945

[9] Interplay Between Primary Cilia and Autophagy and Its Controversial Roles in Cancer [J].

Ko, Je Yeong ;

Lee, Eun Ji ;

Park, Jong Hoon .

BIOMOLECULES & THERAPEUTICS, 2019, 27 (04) :337-341

[10] Role of Tricellular Tight Junction Protein Lipolysis-Stimulated Lipoprotein Receptor (LSR) in Cancer Cells [J].

Kohno, Takayuki ;

Konno, Takumi ;

Kojima, Takashi .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (14)

← 1 2 3 4 →