Three-dimensional architecture of granulosa cell derived from oocyte cumulus complex, revealed by FIB-SEM

被引：3

作者：

Shu, Chongyi ^{[1
]}

Yu, Yiqi ^{[1
]}

Chen, Xiaopan ^{[1
,2
]}

Guo, Jiansheng ^{[3
]}

Zhou, Yier ^{[1
]}

Wu, Dandan ^{[4
]}

Yang, Tianyun ^{[1
]}

Fan, Yuhang ^{[1
]}

Huang, Qiongxiao ^{[1
]}

Shu, Jing ^{[1
]}

机构：

[1] Zhejiang Prov Peoples Hosp, Affiliated Peoples Hosp, Hangzhou Med Coll, Dept Reprod Endocrinol,Ctr Reprod Med Ctr, Hangzhou, Peoples R China

[2] Zhejiang Prov Peoples Hosp, Affiliated Peoples Hosp, Hangzhou Med Coll, Dept Genet & Genom Med, Hangzhou, Peoples R China

[3] Zhejiang Univ, Sch Med, Hangzhou 310058, Peoples R China

[4] Zhejiang Hosp, Dept Obstet, Hangzhou 310012, Peoples R China

来源：

JOURNAL OF OVARIAN RESEARCH | 2023年 / 16卷 / 01期

关键词：

Oocyte cumulus complex; Granulosa cells; Focused ion beam scanning electronmicroscopy; 3D reconstruction; Organelles; Intercellular connections; IN-VITRO; ULTRASTRUCTURE; FERTILIZATION; MEMBRANE; FUSION;

D O I：

10.1186/s13048-023-01298-9

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The oocyte cumulus complex is mainly composed of an oocyte, the perivitelline space, zona pellucida and numerous granulosa cells. The cumulus granulosa cells (cGCs) provide a particularly important microenvironment for oocyte development, regulating its growth, maturation and meiosis. In this study, we studied the internal structures and cell-to-cell connections of mouse cGCs using focused ion beam scanning electron microscopy (FIB-SEM). We reconstructed three-dimensional models to display characteristic connections between the oocyte and cGCs, and to illustrate various main organelles in cGCs together with their interaction relationship. A special form of cilium identified in granulosa cell was never reported in previous literature.

引用

页数：11

共 32 条

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