Expression and Characterization of Dichloromethane Dehalogenase for Dichloromethane Degradation

被引：0
|
作者：
Wu, Yicheng [1 ]
Yu, Chen [1 ]
Fu, Haiyan [1 ]
Yao, Huichao [1 ]
机构：
[1] Xiamen Univ Technol, Coll Environm Sci & Engn, Xiamen, Peoples R China
来源：
PROCEEDINGS OF THE 2022 12TH INTERNATIONAL CONFERENCE ON ENVIRONMENT SCIENCE AND ENGINEERING, ICESE 2022 | 2023年
关键词：
Dichloromethane dehalogenase; Dichloromethane; Pichia pastoris; Enzymatic activity; BIODEGRADATION; MECHANISM;
D O I：
10.1007/978-981-99-1381-7_5
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Dehalogenases widely used to remediate halocarbons contaminated soil. Genetic modification and over expression of recombinant dehalogenases will greatly facilitate its application in environment remediation area. In this study, a gene encoding dehalogenase (LinB) was modificated and over expressed in Pichia pastoris expression system. The results showed that the intracellular recombinant enzymatic activity were most active when the recombinant bacterium cultured at 30 degrees C for 4 h with glycerin 10 g/L as inducer, and the removal rate of 2-choloroethy ethyl sulphide by intracellular enzyme solution was 86.44%.
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收藏
页码：50 / 56
页数：7
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