Embryonic exposure to chloroxylenol induces developmental defects and cardiovascular toxicity via oxidative stress, inflammation, and apoptosis in zebrafish

被引:4
作者
An, Garam [1 ]
Kim, Miji [1 ]
Park, Junho [1 ]
Park, Hahyun [1 ]
Hong, Taeyeon [2 ]
Lim, Whasun [2 ,3 ]
Song, Gwonhwa [1 ,4 ]
机构
[1] Korea Univ, Inst Anim Mol Biotechnol, Coll Life Sci & Biotechnol, Dept Biotechnol, Seoul 02841, South Korea
[2] Sungkyunkwan Univ, Dept Biol Sci, Suwon 16419, South Korea
[3] Sungkyunkwan Univ, Coll Sci, Dept Biol Sci, Suwon 16419, South Korea
[4] Korea Univ, Coll Life Sci & Biotechnol, Dept Biotechnol, Seoul 02841, South Korea
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 2023年 / 268卷
基金
新加坡国家研究基金会;
关键词
Zebrafish; Chloroxylenol; Developmental toxicity; Oxidative stress; Cardiovascular toxicity; ROS; EXPRESSION; TRICLOSAN; DRUGS; HEART; MODEL; WATER;
D O I
10.1016/j.cbpc.2023.109617
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chloroxylenol is an extensively consumed anti-microbial compound. Since its usage is on the rise due to the coronavirus pandemic and ban on other antimicrobial ingredients, recent studies have suggested the necessity of estimating its potential for ecotoxicity. The detrimental effect of chloroxylenol on zebrafish (Danio rerio) viability has been reported; however, research on the mechanisms underlying its toxicity is quite limited. Therefore, we applied the zebrafish model for elucidating responses against chloroxylenol to predict its toxicity toward human health and ecology. Zebrafish exposed to chloroxylenol (0, 0.5, 1, 2.5, 5, and 10 mg/L) at the embryonic stage (from 6 h post-fertilization (hpf) to 96 hpf) showed impaired viability and hatchability, and pathological phenotypes. To address these abnormalities, cellular responses such as oxidative stress, inflammation, and apoptosis were confirmed via in vivo imaging of a fluorescent dye or measurement of the transcriptional changes related to each response. In particular, developmental defects in the cardiovascular system of zebrafish exposed to 0, 0.5, 1, and 2.5 mg/L of chloroxylenol from 6 to 96 hpf were identified by structural analyses of the system in the flk1: eGFP transgenic line. Additional experiments were conducted using human umbilical vein endothelial cells (HUVECs) to predict the adverse impacts of chloroxylenol on the human vascular system. Chloroxylenol impairs the viability and tube formation ability of HUVECs by modulating ERK signaling. The findings obtained using the zebrafish model provide evidence of the possible risks of chloroxylenol exposure and suggest the importance of more in-depth ecotoxicological studies.
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页数:9
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共 58 条
  • [1] The impact of reactive oxygen species in the development of cardiometabolic disorders: a review
    Akhigbe, Roland
    Ajayi, Ayodeji
    [J]. LIPIDS IN HEALTH AND DISEASE, 2021, 20 (01)
  • [2] Environmental impact of COVID-19 pandemic: more negatives than positives
    Ankit
    Kumar, Amit
    Jain, Vartika
    Deovanshi, Ankit
    Lepcha, Ayush
    Das, Chandan
    Bauddh, Kuldeep
    Srivastava, Sudhakar
    [J]. ENVIRONMENTAL SUSTAINABILITY, 2021, 4 (03) : 447 - 454
  • [3] Heart malformation is an early response to TCDD in embryonic zebrafish
    Antkiewicz, DS
    Burns, CG
    Carney, SA
    Peterson, RE
    Heideman, W
    [J]. TOXICOLOGICAL SCIENCES, 2005, 84 (02) : 368 - 377
  • [4] Vortex Dynamics in Trabeculated Embryonic Ventricles
    Battista, Nicholas A.
    Douglas, Dylan R.
    Lane, Andrea N.
    Samsa, Leigh Ann
    Liu, Jiandong
    Miller, Laura A.
    [J]. JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE, 2019, 6 (01)
  • [5] Beamer Brandon, 2010, HSS J, V6, P85, DOI 10.1007/s11420-009-9129-4
  • [6] 2,3,7,8-tetrachlorodibenzo-p-dioxin inhibits regression of the common cardinal vein in developing zebrafish
    Bello, SM
    Heideman, W
    Peterson, RE
    [J]. TOXICOLOGICAL SCIENCES, 2004, 78 (02) : 258 - 266
  • [7] TNF and ROS Crosstal in Inflammation
    Blaser, Heiko
    Dostert, Catherine
    Mak, Tak W.
    Brenner, Dirk
    [J]. TRENDS IN CELL BIOLOGY, 2016, 26 (04) : 249 - 261
  • [8] Neurotoxic effects in zebrafish embryos by valproic acid and nine of its analogues: the fish-mouse connection?
    Brotzmann, Katharina
    Wolterbeek, Andre
    Kroese, Dinant
    Braunbeck, Thomas
    [J]. ARCHIVES OF TOXICOLOGY, 2021, 95 (02) : 641 - 657
  • [9] REACTIVE OXYGEN SPECIES (ROS) - A FAMILY OF FATE DECIDING MOLECULES PIVOTAL IN CONSTRUCTIVE INFLAMMATION AND WOUND HEALING
    Bryan, Nicholas
    Ahswin, Helen
    Smart, Neil
    Bayon, Yves
    Wohlert, Stephen
    Hunt, John A.
    [J]. EUROPEAN CELLS & MATERIALS, 2012, 24 : 249 - 265
  • [10] Antimicrobial agents, triclosan, chloroxylenol, methylisothiazolinone and borax, used in cleaning had genotoxic and histopathologic effects on rainbow trout
    Capkin, Erol
    Ozcelep, Tuna
    Kayis, Sevki
    Altinok, Ilhan
    [J]. CHEMOSPHERE, 2017, 182 : 720 - 729