Adaptation of CUT&RUN for use in African trypanosomes

被引：0

作者：

Miller, Geneva ^{[1
]}

Rollosson, Lindsey M. ^{[1
]}

Saada, Carrie ^{[1
]}

Wade, Serenity J. ^{[1
]}

Schulz, Danae ^{[1
]}

机构：

[1] Harvey Mudd Coll, Claremont, CA 91711 USA

来源：

PLOS ONE | 2023年 / 18卷 / 11期

基金：

美国国家科学基金会;

关键词：

HISTONE MODIFICATIONS; GENE-EXPRESSION; CHROMATIN; BIAS;

D O I：

10.1371/journal.pone.0292784

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This Cleavage Under Targets and Release Using Nuclease (CUT&RUN) protocol produces genomic occupancy data for a protein of interest in the protozoan parasite Trypanosoma brucei. The data produced is analyzed in a similar way as that produced by ChIP-seq. While we describe the protocol for parasites carrying an epitope tag for the protein of interest, antibodies against the native protein could be used for the same purpose.

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页数：7

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