Adaptation of CUT&RUN for use in African trypanosomes

被引：0
作者：
Miller, Geneva [1 ]
Rollosson, Lindsey M. [1 ]
Saada, Carrie [1 ]
Wade, Serenity J. [1 ]
Schulz, Danae [1 ]
机构：
[1] Harvey Mudd Coll, Claremont, CA 91711 USA
来源：
PLOS ONE | 2023年 / 18卷 / 11期
基金：
美国国家科学基金会;
关键词：
HISTONE MODIFICATIONS; GENE-EXPRESSION; CHROMATIN; BIAS;
D O I：
10.1371/journal.pone.0292784
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
This Cleavage Under Targets and Release Using Nuclease (CUT&RUN) protocol produces genomic occupancy data for a protein of interest in the protozoan parasite Trypanosoma brucei. The data produced is analyzed in a similar way as that produced by ChIP-seq. While we describe the protocol for parasites carrying an epitope tag for the protein of interest, antibodies against the native protein could be used for the same purpose.
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页数：7
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