Abnormal expression and related regulatory mechanism of long noncoding RNA in head and neck squamous cell carcinoma

Objective. The objective of this study was to investigate the effect and mechanism of long noncoding RNA (lncRNA) on head and neck squamous cell carcinoma (HNSCC).Study Design. RNA was extracted from HNSCC tissue and adjacent tissues and sequenced. Differentially expressed lncRNA and microRNA (miRNA) were screened. Quantitative real-time polymerase chain reaction was used to compare the expression of tar-get lncRNA between human oral keratinocyte (HOK) and cancer cell lines. LAMB1-210 in SCC-4 and CAL-27 cells was downregulated by transfecting short hairpin RNA (shRNA). Methyl thiazolyl tetrazolium, wound healing, and Transwell assay were used to evaluate viability, migration, and invasion ability in SCC-4 and CAL-27 cells.Results. Eighty-one lncRNAs were differentially expressed, of which 40 were upregulated, 41 were downregulated, and 280 miRNAs were differentially expressed. Compared with HOK cells, the expression of LAMB 1-210 was significantly upregulated in FADU, SCC-4, and CAL-27 cells (P < .001). LAMB1-210 was successfully downregulated by shRNA-LAMB1-210 (P < .001), and downregulated LAMB1-210 inhibited the cell viability, migration, and invasion ability of SCC-4 and CAL-27 cells (P < .001).Conclusions. The expression of lncRNA and miRNA in HNSCC tissues differs from that in normal tissues. LAMB1-210 is significantly overexpressed in tumor cells and is related to their survival, migration, and invasion.