Gas Chromatography-Mass Spectrometry-Based Cerebrospinal Fluid Metabolomics to Reveal the Protection of Coptisine against Transient Focal Cerebral Ischemia-Reperfusion Injury via Anti-Inflammation and Antioxidant

被引：7

作者：

Zhang, Junjie ^{[1
,2
,3
]}

Qi, Ao ^{[2
,3
]}

Liu, Lulu ^{[2
,3
]}

Cai, Chun ^{[2
,3
]}

Xu, Hui ^{[1
]}

机构：

[1] Guangzhou Univ Chinese Med, Res Ctr Chinese Herbal Resource Sci & Engn, Key Lab Chinese Med Resource Lingnan, Minist Educ,Sch Tradit Chinese Meteria Med, Guangzhou 510006, Peoples R China

[2] Southern Marine Sci & Engn Guangdong Lab, Zhanjiang 524023, Peoples R China

[3] Guangdong Med Univ, Sch Pharm, Zhanjiang 524023, Peoples R China

来源：

MOLECULES | 2023年 / 28卷 / 17期

基金：

中国国家自然科学基金;

关键词：

cerebral ischemia-reperfusion; Coptisine; arachidonic acid metabolomics; inflammation; oxidative stress; 8-ISO-PROSTAGLANDIN F2-ALPHA; STROKE; INHIBITION; DISEASE;

D O I：

10.3390/molecules28176350

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Coptisine (Cop) exerts a neuroprotective effect on central nervous system disease, particularly ischemic stroke. However, its protective mechanism is still unclear. This study aimed to investigate the protective effect of Cop on cerebral ischemia-reperfusion (IR) rats with a middle cerebral artery occlusion model by integrating a gas chromatography-mass spectrometry (GC-MS)-based metabolomics approach with biochemical assessment. Our results showed that Cop could improve neurobehavioral function and decrease the ischemia size in IR rats. In addition, Cop was found to decrease inflammatory mediators (e.g., prostaglandin D2 (PGD2) and tumor necrosis factor-& alpha; (TNF-& alpha;) and attenuate oxidative stress response (e.g., increase the superoxide dismutase (SOD) expression and decrease 8-iso-PGF2 & alpha; level). Furthermore, the GC-MS-based cerebrospinal fluid (CSF) metabolomics analysis indicated that Cop influenced the level of glycine, 2,3,4-trihydroxybutyric acid, oleic acid, glycerol, and ribose during IR injury. Cop exhibited a good neuroprotective effect against cerebral IR injury and metabolic alterations, which might be mediated through its antioxidant and anti-inflammatory properties.

引用

页数：12

共 43 条

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