Detection of human serum albumin using a rare-earth nanosheet fluorescent probe based on intensity and lifetime signals

被引：1

作者：

Liu, Susu ^{[1
]}

Xia, Jinfeng ^{[2
]}

Jiang, Danyu ^{[2
]}

Li, Qiang ^{[1
]}

Pan, Xin ^{[1
]}

Dong, Xueting ^{[1
]}

Wang, Jiuxia ^{[1
]}

Liu, Yue ^{[1
]}

Dong, Yuchen ^{[1
]}

机构：

[1] East China Normal Univ, Sch Chem & Mol Engn, 3663 Zhongshanbei Rd, Shanghai 200062, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Ceram, 1295 Dingxi Rd, Shanghai 200050, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2023年 / 302卷

关键词：

Fluorescent probe; Nanosheet; Lifetime; HSA; SYSTEM;

D O I：

10.1016/j.saa.2023.123046

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Human serum albumin (HSA) is an important biomarker for early disease diagnosis. Therefore, the detection of HSA in biological samples is important. In this study, for the sensitive detection of HSA, a fluorescent probe based on Eu(III)-doped yttrium hydroxide nanosheets was designed and sensitized by & alpha;-thiophenformyl acetone trifluoride as an antenna. The morphology and structure of the as-prepared nanosheet fluorescent probe were studied by transmission electron microscopy and atomic force microscopy. A detailed investigation of the fluorescence properties of the as-obtained nanosheet probe revealed that the Eu(III) emission intensity was linearly and selectively enhanced by the consecutive addition of HSA. Furthermore, the lifetime signal of this probe was enhanced with increasing concentration. The sensitivity of the nanosheet probe to HSA is discussed based on the results of ultraviolet-visible, fluorescence, and infrared spectral analyses, the findings demonstrate that the nanosheet fluorescent probe we prepared is a highly sensitive and selective tool for detecting HSA concentration with a high intensity and a large lifetime change.

引用

页数：9