The Impact of Cancer-Associated Fibroblasts on Drug Resistance, Stemness, and Epithelial-Mesenchymal Transition in Bladder Cancer: A Comparison between Recurrent and Non-Recurrent Patient-Derived CAFs

被引：11

作者：

Rastegar-Pouyani, Nima ^{[1
]}

Montazeri, Vahideh ^{[2
]}

Marandi, Nikoo ^{[3
]}

Aliebrahimi, Shima ^{[2
]}

Andalib, Melika ^{[4
]}

Jafarzadeh, Emad ^{[1
]}

Montazeri, Hamed ^{[4
,7
]}

Ostad, Seyed Nasser ^{[1
,5
,6
]}

机构：

[1] Univ Tehran Med Sci, Dept Pharmacol & Toxicol, Tehran, Iran

[2] Smart Univ Med Sci, Dept Artificial Intelligence, Tehran, Iran

[3] Islamic Azad Univ Med Sci, Sch Pharm, Tehran, Iran

[4] Iran Univ Med Sci, Sch Pharm, Dept Pharmacognosy & Pharmaceut Biotechnol, Tehran, Iran

[5] Univ Tehran Med Sci, Toxicol & Poisoning Res Ctr, Dept Toxicol & Pharmacol, Tehran, Iran

[6] Univ Tehran Med Sci, Fac Pharm, Dept Pharmacol & Toxicol, Tehran, Iran

[7] Iran Univ Med Sci, Sch Pharm, Dept Pharmacognosy & Pharmaceut Biotechnol, Tehran, Iran

来源：

CANCER INVESTIGATION | 2023年 / 41卷 / 07期

关键词：

Cancer-associated fibroblast; bladder cancer; cancer stem cell; epithelial-mesenchymal transition; co-culture; TUMOR MICROENVIRONMENT; POOR-PROGNOSIS; CELLS; IDENTIFICATION; ACTIVATION; PROMOTE; SNAIL; EMT;

D O I：

10.1080/07357907.2023.2237576

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

This study comparatively evaluated the possible effects of recurrent and non-recurrent patient-derived Cancer-Associated Fibroblasts (CAFs-R and -NR) on the bladder cancer cell line, EJ138. Both groups of CAFs increased cisplatin resistance and altered cell cycle distribution alongside induced resistance to apoptosis. Later, the scratch assay confirmed the cell migration-inducing effects of CAFs on cells. Nonetheless, only CAFs-R managed to increase sphere-formation and clonogenic levels in EJ138 cells, which were later validated by upregulating pluripotency transcription factors. Besides, CAFs-R also affected the expression levels of some of the EMT markers. Our study suggests that CAFs-R had stronger pro-tumorigenic effects on EJ138 cells.

引用

页码：656 / 671

页数：16

共 47 条

[31] LTBP1 promotes esophageal squamous cell carcinoma progression through epithelial-mesenchymal transition and cancer-associated fibroblasts transformation
Cai, Rui
Wang, Ping
Zhao, Xin
Lu, Xiansheng
Deng, Ruxia
Wang, Xiaoyu
Su, Zhaoji
Hong, Chang
Lin, Jie
JOURNAL OF TRANSLATIONAL MEDICINE, 2020, 18 (01)
[32] Galectin-1 from cancer-associated fibroblasts induces epithelial-mesenchymal transition through β1 integrin-mediated upregulation of Gli1 in gastric cancer
Chong, Yang
Tang, Dong
Xiong, Qingquan
Jiang, Xuetong
Xu, Chuanqi
Huang, Yuqin
Wang, Jie
Zhou, Huaicheng
Shi, Youquan
Wu, Xiaoqing
Wang, Daorong
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2016, 35 : 1 - 13
[33] TGF-β1-activated cancer-associated fibroblasts promote breast cancer invasion, metastasis and epithelial-mesenchymal transition by autophagy or overexpression of FAP-α
Huang, Minli
Fu, Mengru
Wang, Jia
Xia, Chunhua
Zhang, Hong
Xiong, Yuqing
He, Jiake
Liu, Jianming
Liu, Bingchen
Pan, Siyi
Liu, Fanglan
BIOCHEMICAL PHARMACOLOGY, 2021, 188
[34] IL-6 Secreted from Cancer-Associated Fibroblasts Mediates Chemoresistance in NSCLC by Increasing Epithelial-Mesenchymal Transition Signaling
Shintani, Yasushi
Fujiwara, Ayako
Kimura, Toru
Kawamura, Tomohiro
Funaki, Soichiro
Minami, Masato
Okumura, Meinoshin
JOURNAL OF THORACIC ONCOLOGY, 2016, 11 (09) : 1482 - 1492
[35] Patient-Derived, Drug-Resistant Colon Cancer Cells Evade Chemotherapeutic Drug Effects via the Induction of Epithelial-Mesenchymal Transition-Mediated Angiogenesis
Lim, Jin Hong
Choi, Kyung Hwa
Kim, Soo Young
Park, Cheong Soo
Kim, Seok-Mo
Park, Ki Cheong
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (20) : 1 - 16
[36] Single-cell and multi-omics analyses highlight cancer-associated fibroblasts-induced immune evasion and epithelial mesenchymal transition for smoking bladder cancer
Wu, Jiajin
Gao, Fang
Meng, Rui
Li, Huiqin
Mao, Zhenguang
Xiao, Yanping
Pu, Qiuyi
Du, Mulong
Zhang, Zhengdong
Shao, Qiang
Zheng, Rui
Wang, Meilin
TOXICOLOGY, 2024, 504
[37] WDR5 drives the development of cervical squamous cell carcinoma by inducing epithelial-mesenchymal transition and cancer-associated fibroblasts formation
Sun, Fangli
Mo, Linmei
Lan, Ying
Lu, Qiuping
Wu, Nengxian
Song, Honglin
PATHOLOGY RESEARCH AND PRACTICE, 2022, 238
[38] Cancer-associated fibroblasts promote epithelial-mesenchymal transition and EGFR-TKI resistance of non-small cell lung cancers via HGF/IGF-1/ANXA2 signaling
Yi, Yanmei
Zeng, Shanshan
Wang, Zhaotong
Wu, Minhua
Ma, Yuanhuan
Ye, Xiaoxia
Zhang, Biao
Liu, Hao
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 2018, 1864 (03): : 793 - 803
[39] CD248-expressing cancer-associated fibroblasts induce epithelial-mesenchymal transition of non-small cell lung cancer via inducing M2-polarized macrophages
Xiao, Jing
Yang, Zeyang
Wang, Siyu
Liu, Xinlei
Wang, Yun
Hu, Zuquan
Zeng, Zhu
Wu, Jieheng
SCIENTIFIC REPORTS, 2024, 14 (01):
[40] Interfering HMGB3 release from cancer-associated fibroblasts by miR-200b represses chemoresistance and epithelial-mesenchymal transition of gastric cancer cells
Ke, Yanzhuang
Mai, Jieying
Liu, Zhendong
Xu, Yuyang
Zhao, Chunyi
Wang, Baochun
JOURNAL OF GASTROINTESTINAL ONCOLOGY, 2022, 13 (05) : 2197 - +

← 1 2 3 4 5 →