The Impact of Cancer-Associated Fibroblasts on Drug Resistance, Stemness, and Epithelial-Mesenchymal Transition in Bladder Cancer: A Comparison between Recurrent and Non-Recurrent Patient-Derived CAFs

被引：11
|
作者：
Rastegar-Pouyani, Nima [1 ]
Montazeri, Vahideh [2 ]
Marandi, Nikoo [3 ]
Aliebrahimi, Shima [2 ]
Andalib, Melika [4 ]
Jafarzadeh, Emad [1 ]
Montazeri, Hamed [4 ,7 ]
Ostad, Seyed Nasser [1 ,5 ,6 ]
机构：
[1] Univ Tehran Med Sci, Dept Pharmacol & Toxicol, Tehran, Iran
[2] Smart Univ Med Sci, Dept Artificial Intelligence, Tehran, Iran
[3] Islamic Azad Univ Med Sci, Sch Pharm, Tehran, Iran
[4] Iran Univ Med Sci, Sch Pharm, Dept Pharmacognosy & Pharmaceut Biotechnol, Tehran, Iran
[5] Univ Tehran Med Sci, Toxicol & Poisoning Res Ctr, Dept Toxicol & Pharmacol, Tehran, Iran
[6] Univ Tehran Med Sci, Fac Pharm, Dept Pharmacol & Toxicol, Tehran, Iran
[7] Iran Univ Med Sci, Sch Pharm, Dept Pharmacognosy & Pharmaceut Biotechnol, Tehran, Iran
来源：
CANCER INVESTIGATION | 2023年 / 41卷 / 07期
关键词：
Cancer-associated fibroblast; bladder cancer; cancer stem cell; epithelial-mesenchymal transition; co-culture; TUMOR MICROENVIRONMENT; POOR-PROGNOSIS; CELLS; IDENTIFICATION; ACTIVATION; PROMOTE; SNAIL; EMT;
D O I：
10.1080/07357907.2023.2237576
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
This study comparatively evaluated the possible effects of recurrent and non-recurrent patient-derived Cancer-Associated Fibroblasts (CAFs-R and -NR) on the bladder cancer cell line, EJ138. Both groups of CAFs increased cisplatin resistance and altered cell cycle distribution alongside induced resistance to apoptosis. Later, the scratch assay confirmed the cell migration-inducing effects of CAFs on cells. Nonetheless, only CAFs-R managed to increase sphere-formation and clonogenic levels in EJ138 cells, which were later validated by upregulating pluripotency transcription factors. Besides, CAFs-R also affected the expression levels of some of the EMT markers. Our study suggests that CAFs-R had stronger pro-tumorigenic effects on EJ138 cells.
引用
收藏
页码：656 / 671
页数：16
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