An ingenious designed dual mode self-powered biosensing platform based on graphdiyne heterostructure substrate for instant hepatocarcinoma marker detection

被引：16

作者：

Xu, Jing ^{[1
]}

Liu, Yinbing ^{[1
]}

Huang, Ke-Jing ^{[2
]}

Wang, Renjie ^{[3
]}

Sun, Xiaoxuan ^{[1
]}

机构：

[1] Xinyang Normal Univ, Coll Chem & Chem Engn, Xinyang 464000, Peoples R China

[2] Guangxi Minzu Univ, Sch Chem & Chem Engn, Key Lab Chem & Engn Forest Prod, State Ethn Affairs Commiss,Guangxi Key Lab Chem &, Nanning 530006, Peoples R China

[3] Florida Atlantic Univ, Dept Chem & Biochem, Boca Raton, FL 33431 USA

来源：

TALANTA | 2023年 / 261卷

关键词：

Graphdiyne heterojunction; Dual-mode real-time detection; Self-powered biosensing platform; Cascade amplification;

D O I：

10.1016/j.talanta.2023.124656

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report here for the first time a self-powered biosensing platform based on graphene/graphdiyne/graphene (GDY-Gr) heterostructure substrate material for ultrasensitive hepatocarcinoma marker (microRNA-21) detec-tion in both electrochemical and colorimetric test modes. The dual-mode signal intuitively displayed on a smartphone fundamentally improves the detection accuracy. In electrochemical mode, the calibration curve is established in the linear range of 0.1-10000 fM, and the detection limit is as low as 0.333 fM (S/N = 3). Simultaneously, colorimetric analysis of the miRNA-21 is realized by using ABTS as an indicator. The detection limit is confirmed as 32 fM (S/N = 3), and miRNA-21 of concentration from 0.1 pM to 1 nM exhibit a linear relationship with R2 = 0.9968. Overall, the combination of GDY-Gr and multiple signal amplification strategy significantly improved the sensitivity by 310 times compared with traditional enzymatic biofuel cells (EBFCs) based detection platform, showing broad application prospects for on-site analysis and future mobile medical services.

引用

页数：8