Effects of fermentation time on the bioactive constituents of Kinema, a traditional fermented food of Nepal

Kinema is a traditional food prepared by the natural fermentation of cooked soybeans. The fer-mented Kinema is known to have several bioactive constituents, however, only limited reports on the effect of fermentation time on the bioactivity of Kinema are available. Therefore, in this work, changes in phenolics content and radical scavenging activity of Kinema at different fermentation times were explored. Furthermore, the optimum fermentation time for maximum bioactivities (total phenolic content, total flavonoid content, and DPPH radical scavenging activity) was determined using one-factor response surface methodology. The numerical optimization sug-gested an optimum fermentation time of 29.6 h with significantly higher total phenolics and flavonoid contents of 62.84 +/- 0.89 mg GAEs/g dry extract, 45.41 +/- 0.57 mg QEs/g dry extract, respectively (p < 0.05) compared to traditionally fermented Kinema. Similarly, the IC50 con-centration for DPPH radical scavenging activity of 1.78 +/- 0.01 mg dry extract/mL, was signifi-cantly lower than those for traditionally prepared Kinema (p < 0.05). Moreover, optimized Kinema had significantly higher overall sensory scores compared to the traditional sample. The results suggested that fermentation time affects the amount of bioactive constituents of Kinema. Further studies are needed to explore the changes in the type of phenolic and flavonoid compounds.