Bacterial diversity of an acid mine drainage beside the Xichu River (Mexico) accessed by culture-dependent and culture-independent approaches

被引:1
作者
Brito, Elcia Margareth Souza [1 ]
Guyoneaud, Remy [2 ]
Caretta, Cesar Augusto [3 ]
Joseph, Manon [4 ]
Goni-Urriza, Marisol [2 ]
Ollivier, Bernard [4 ]
Hirschler-Rea, Agnes [4 ]
机构
[1] Univ Guanajuato, Environm Engn Dept, Lab Environm Microbiol & Appl Mol Biol, DI CGT, Guanajuato 36000, Gto, Mexico
[2] Univ Pau & Pays Adour, UMR 5254, Environm Microbiol Grp, IPREM,E2S UPPA,CNRS, Pau, France
[3] Univ Guanajuato, Astron Dept, DCNE CGT, Guanajuato 36023, Gto, Mexico
[4] Aix Marseille Univ, Inst Mediterraneen Oceanol MIO, UM 110, CNRS,IRD, Marseille, France
关键词
Sulfate-reducing bacteria; Arsenate-reducing bacteria; Raineyella; Metals; MiSEQ; Acid mine drainage; SULFATE-REDUCING BACTERIA; SP-NOV; GEN; NOV; MICROBIAL COMMUNITIES; STRAIN; REDUCTION; ECOLOGY; BIOFILM; BACILLARIOPHYTA; RESPIRATION;
D O I
10.1007/s00792-023-01291-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Xichu River is a Mexican river located in an environmental preservation area called Sierra Gorda Biosphere Reserve. Around it, there are tons of abandoned mine residues that represent a serious environmental issue. Sediment samples of Xichu River, visibly contaminated by flows of an acid mine drainage, were collected to study their prokaryotic diversity. The study was based on both cultural and non-cultural approaches. The analysis of total 16S rRNA gene by MiSEQ sequencing allowed to identify 182 Operational Taxonomic Units. The community was dominated by Pseudomonadota, Bacteroidota, "Desulfobacterota" and Acidobacteriota (27, 21, 19 and 16%, respectively). Different culture conditions were used focusing on the isolation of anaerobic bacteria, including sulfate-reducing bacteria (SRB) and arsenate-reducing bacteria (ARB). Finally, 16 strains were isolated. Among them, 12 were phylogenetically identified, with two strains being SRB, belonging to the genus Solidesulfovibrio ("Desulfobacterota"), while ten are ARB belonging to the genera Azospira (Pseudomonadota), Peribacillus (Bacillota), Raineyella and Propionicimonas (Actinomycetota). The isolate representative of Raineyella genus probably corresponds to a new species, which, besides arsenate, also reduces nitrate, nitrite, and fumarate.
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页数:16
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