Direct quantification of cytosolic delivery of drug nanocarriers using FlAsH-EDT2

被引：2

作者：

Rotem, R. ^{[1
]}

Prosperi, D. ^{[1
]}

Bertolini, J. A. ^{[1
]}

Salvioni, L. ^{[1
]}

Barbieri, L. ^{[1
]}

Rizzuto, M. A. ^{[1
]}

Tinelli, V. ^{[1
]}

Gori, A. ^{[2
]}

Adams, S. ^{[3
]}

Colombo, M. ^{[1
]}

机构：

[1] Univ Milano Bicocca, Milan, Italy

[2] Ist Sci & tecnol Chim, Milan, Italy

[3] Univ Calif San Diego, La Jolla, CA 92093 USA

来源：

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | 2023年 / 47卷

关键词：

Quantification method; Cell penetration; Drug delivery; FlAsH-EDT2; Flow cytometry; Introduction; PROTEIN; ENTRY;

D O I：

10.1016/j.nano.2022.102626

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The delivery of therapeutics across the cell membrane and into the cytoplasm is a major challenge that limits the development of new therapies. This challenge is compounded by the lack of a general assay for cytosolic delivery. Here we develop this assay based on the profluorophore CrAsH-EDT2, and provide cytosolic penetration results for a variety of drug delivery agents (polyethyleneimine, poly-arginine, Ferritin, poly [maleic anhydride-alt-isobutene] grafted with dodecylamine, and cationic liposomes) into HeLa and T98G cells. Our results show that this method can be widely applicable to different cells and drug delivery agents, and yield statistically robust results. We later use this method to optimize and improve a model drug delivery agent's (Ferritin) cytosolic penetration. (c) 2022 Elsevier Inc. All rights reserved.

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页数：11

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