Metabolites analysis and new bioactive pigments from a Monascus purpureus strain with low citrinin

被引：3

作者：

Huang, Yongfang ^{[1
]}

Fu, Ruiyan ^{[2
]}

Yin, Wenxuan ^{[1
]}

Chen, Renjie ^{[1
]}

Zhao, Cheng ^{[1
]}

Hu, Fenglin ^{[1
]}

Bao, Guanhu ^{[3
]}

Lu, Ruili ^{[1
]}

机构：

[1] Anhui Agr Univ, Engn Res Ctr Fungal Biotechnol, Minist Educ, Hefei 230036, Peoples R China

[2] Anhui Agr Univ, Sch Tea & Food Sci & Technol, Hefei 230036, Peoples R China

[3] Anhui Agr Univ, Nat Prod Lab, State Key Lab Tea Plant Biol & Utilizat, Hefei 230036, Anhui Prov, Peoples R China

来源：

MICROCHEMICAL JOURNAL | 2023年 / 195卷

基金：

中国国家自然科学基金;

关键词：

Monascus purpureus; Metabolites; Structure identification; Anti-inflammatory; Cell proliferation; RED YEAST RICE; ANTIINFLAMMATORY ACTIVITY; BIOSYNTHESIS; DERIVATIVES; YELLOW; ORANGE;

D O I：

10.1016/j.microc.2023.109441

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A Monascus purpureus strain with high mycelial biomass and no detected citrinin in liquid fermentation was selected from 20 strains. HPLC-DAD-HRMS analysis revealed that the EtOAc extract of the fermented mash contained 2 unknown compounds and 11 known metabolites of Monascus fungi. Isolations with preparative and semi-preparative HPLC resulted in four brownish red pigments. The two unknown pigments were identified with HRMS, CD, ECD, 1D and 2D NMR, and designated as monascuskaolin A (3) and B (8). The two known com-pounds were verified with 13C NMR as monascusazaphilone C and monasfluol B. Result of bioactivity tests showed that the two new pigments were able to significantly reduce the transcription levels of IL-1 beta, TNF-alpha, and TGF-beta, and significantly promote the proliferation of 293 T cells, suggesting that they have anti-inflammatory function and may be good for human kidney. Metabolites studies suggested that M. purpureus RCEF7056 is a safe strain with functional food utilization potential.

引用

页数：8

共 44 条

[1] Anti-tumor-initiating effects of monascin, an azaphilonoid pigment from the extract of Monascus pilosus fermented rice (red-mold rice) [J].

Akihisa, T ;

Tokuda, H ;

Ukiya, M ;

Kiyota, A ;

Yasukawa, K ;

Sakamoto, N ;

Kimura, Y ;

Suzuki, T ;

Takayasu, J ;

Nishino, H .

CHEMISTRY & BIODIVERSITY, 2005, 2 (10) :1305-1309

[2] DISCOVERY, BIOCHEMISTRY AND BIOLOGY OF LOVASTATIN [J].

ALBERTS, AW .

AMERICAN JOURNAL OF CARDIOLOGY, 1988, 62 (15) :J10-J15

[3] Nature's Cholesterol-Lowering Drug: Isolation and Structure Elucidation of Lovastatin from Red Yeast Rice-Containing Dietary Supplements [J].

不详 .

JOURNAL OF CHEMICAL EDUCATION, 2012, 89 (01) :138-140

[4] Mpp7 controls regioselective Knoevenagel condensation during the biosynthesis of Monascus azaphilone pigments [J].

Balakrishnan, Bijinu ;

Chen, Chien-Chi ;

Pan, Tzu-Ming ;

Kwon, Hyung-Jin .

TETRAHEDRON LETTERS, 2014, 55 (09) :1640-1643

[5] Small molecule compounds with good anti-inflammatory activity reported in the literature from 01/2009 to 05/2021: a review [J].

Bian, Ming ;

Ma, Qian-qian ;

Wu, Yun ;

Du, Huan-huan ;

Guo-hua, Gong .

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, 2021, 36 (01) :2139-2159

[6] PRODUCTION OF CITRININ BY VARIOUS SPECIES OF MONASCUS [J].

BLANC, PJ ;

LORET, MO ;

GOMA, G .

BIOTECHNOLOGY LETTERS, 1995, 17 (03) :291-294

[7] The landscape of aging [J].

Cai, Yusheng ;

Song, Wei ;

Li, Jiaming ;

Jing, Ying ;

Liang, Chuqian ;

Zhang, Liyuan ;

Zhang, Xia ;

Zhang, Wenhui ;

Liu, Beibei ;

An, Yongpan ;

Li, Jingyi ;

Tang, Baixue ;

Pei, Siyu ;

Wu, Xueying ;

Liu, Yuxuan ;

Zhuang, Cheng-Le ;

Ying, Yilin ;

Dou, Xuefeng ;

Chen, Yu ;

Xiao, Fu-Hui ;

Li, Dingfeng ;

Yang, Ruici ;

Zhao, Ya ;

Wang, Yang ;

Wang, Lihui ;

Li, Yujing ;

Ma, Shuai ;

Wang, Si ;

Song, Xiaoyuan ;

Ren, Jie ;

Zhang, Liang ;

Wang, Jun ;

Zhang, Weiqi ;

Xie, Zhengwei ;

Qu, Jing ;

Wang, Jianwei ;

Xiao, Yichuan ;

Tian, Ye ;

Wang, Gelin ;

Hu, Ping ;

Ye, Jing ;

Sun, Yu ;

Mao, Zhiyong ;

Kong, Qing-Peng ;

Liu, Qiang ;

Zou, Weiguo ;

Tian, Xiao-Li ;

Xiao, Zhi-Xiong ;

Liu, Yong ;

Liu, Jun-Ping .

SCIENCE CHINA-LIFE SCIENCES, 2022, 65 (12) :2354-2454

[8] Natural pigment from Monascus: The production and therapeutic significance [J].

Chaudhary V. ;

Katyal P. ;

Poonia A.K. ;

Kaur J. ;

Puniya A.K. ;

Panwar H. .

Journal of Applied Microbiology, 2022, 133 (01) :18-38

[9]

Chen J, 2022, FOOD FUNCT, V13, P7000, DOI [10.1039/d2fo00402j, 10.1039/D2FO00402J]

[10] Nature and nurture: confluence of pathway determinism with metabolic and chemical serendipity diversifies Monascus azaphilone pigments [J].

Chen, Wanping ;

Feng, Yanli ;

Molnar, Istvan ;

Chen, Fusheng .

NATURAL PRODUCT REPORTS, 2019, 36 (04) :561-572

← 1 2 3 4 5 →