Evaluation of secretomes derived from human dermal and adipose tissue mesenchymal stem/stromal cells for skin wound healing: not as effective as cells

被引:2
作者
Zomer, Helena Debiazi [1 ,2 ]
de Souza Lima, Victor Juan [2 ]
Bion, Monique Coelho [2 ,3 ]
Brito, Karynne Nazare Lins [2 ]
Rode, Michele [2 ]
Stimamiglio, Marco Augusto [4 ]
Jeremias, Talita da Silva [2 ]
Trentin, Andrea Goncalves [2 ,5 ]
机构
[1] Univ Florida, Dept Physiol Sci, Gainesville, FL 32611 USA
[2] Univ Fed Santa Catarina, Dept Cell Biol Embryol & Genet, Florianopolis, SC, Brazil
[3] Fed Univ Rio Janeiro, Natl Inst Translat Neurosci, Rio De Janeiro, Brazil
[4] FIOCRUZ-PR, Carlos Chagas Inst, Lab Stem Cells Basic Biol, Curitiba, PR, Brazil
[5] Natl Inst Sci & Technol Regenerat Med, Rio De Janeiro, Brazil
关键词
MSC; Dermis; Conditioned medium; Exosomes; Integra matrix; Proteomic; STEM-CELLS; CONDITIONED MEDIUM; STROMAL CELLS; EXTRACELLULAR VESICLES; ENHANCE ANGIOGENESIS; GROWTH-FACTORS; MATRIX; REPAIR;
D O I
10.1186/s13287-023-03630-y
中图分类号
Q813 [细胞工程];
学科分类号
摘要
BackgroundAlthough the paracrine effects of mesenchymal stem/stromal cells (MSCs) have been recognized as crucial mediators of their regenerative effects on tissue repair, the potential of MSC secretomes as effective substitutes for cellular therapies remains underexplored.MethodsIn this study, we compared MSCs from the human dermis (DSCs) and adipose tissue (ASCs) with their secretomes regarding their efficacy for skin wound healing using a translationally relevant murine model.ResultsProteomic analysis revealed that while there was a substantial overlap in protein composition between DSC and ASC secretomes, specific proteins associated with wound healing and angiogenesis were differentially expressed. Despite a similar angiogenic potential in vivo, DSC and ASC secretomes were found to be less effective than cells in accelerating wound closure and promoting tissue remodeling.ConclusionsOverall, secretome-treated groups showed intermediary results between cells- and control-treated (empty scaffold) groups. These findings highlight that although secretomes possess therapeutic potential, their efficacy might be limited compared to cellular therapies. This study contributes to the growing understanding of MSC secretomes, emphasizes the need for further protocol optimization, and offers insights into their potential applications in regenerative medicine.
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页数:14
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