Volumetric Absorptive Microsampling in the Analysis of Endogenous Metabolites

被引：9

作者：

de Sa e Silva, Daniel Marques ^{[1
,2
]}

Thaitumu, Marlene ^{[2
,3
]}

Theodoridis, Georgios ^{[1
,2
]}

Witting, Michael ^{[4
,5
]}

Gika, Helen ^{[2
,3
]}

机构：

[1] Aristotle Univ Thessaloniki AUTH, Dept Chem, Thessaloniki 54124, Greece

[2] Ctr Interdisciplinary Res & Innovat CIRI AUTH, Biomic AUTh, Balkan Ctr B1 4, GR-57001 Thessaloniki, Greece

[3] Aristotle Univ Thessaloniki, Sch Med, Thessaloniki 54124, Greece

[4] Helmholtz Zentrum Munchen, Metab & Proteom Core, Ingolstadter Land Str 1, D-85764 Neuherberg, Germany

[5] Tech Univ Munich, TUM Sch Life Sci, Chair Analyt Food Chem, Maximus von Imhof Forum 6, D-85354 Freising Weihenstephan, Germany

来源：

METABOLITES | 2023年 / 13卷 / 10期

基金：

欧盟地平线“2020”;

关键词：

volumetric absorptive microsampling (VAMS); blood microsampling; endogenous metabolites; biomarkers; analytical validation; clinical application; WHOLE-BLOOD; QUANTITATIVE BIOANALYSIS; LC-MS/MS; THIAMINE STATUS; HEMATOCRIT; DEVICES; PLASMA; VAMS; QUANTIFICATION; VALIDATION;

D O I：

10.3390/metabo13101038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Volumetric absorptive microsampling (VAMS) has arisen as a relevant tool in biological analysis, offering simplified sampling procedures and enhanced stability. Most of the attention VAMS has received in the past decade has been from pharmaceutical research, with most of the published work employing VAMS targeting drugs or other exogenous compounds, such as toxins and pollutants. However, biomarker analysis by employing blood microsampling has high promise. Herein, a comprehensive review on the applicability of VAMS devices for the analysis of endogenous metabolites/biomarkers was performed. The study presents a full overview of the analysis process, incorporating all the steps in sample treatment and validation parameters. Overall, VAMS devices have proven to be reliable tools for the analysis of endogenous analytes with biological importance, often offering improved analyte stability in comparison with blood under ambient conditions as well as a convenient and straightforward sample acquisition model.

引用

页数：24

共 54 条

[21] Opportunities and obstacles for microsampling techniques in bioanalysis: Special focus on DBS and VAMS [J].