Molecular epidemiology of Bartonella species from sympatric mammals collected in urban and rural areas of Punjab, Pakistan

被引:3
作者
Rizwan, Muhammad [1 ,2 ]
Ali, Shahzad [1 ,2 ]
Javid, Arshad [2 ]
von Fricken, Michael E. [3 ]
Rashid, Muhammad Imran [4 ]
机构
[1] Univ Vet & Anim Sci, Dept Wildlife & Ecol, Wildlife Epidemiol & Mol Microbiol Lab, Discipline Zool,One Hlth Res Grp, Ravi Campus, Lahore, Pattoki, Pakistan
[2] Univ Vet & Anim Sci, Dept Wildlife & Ecol, Ravi Campus, Lahore, Pattoki, Pakistan
[3] George Mason Univ, Dept Global & Community Hlth, Fairfax, VA USA
[4] Univ Vet & Anim Sci, Dept Parasitol, Lahore, Pakistan
关键词
Bartonella; Rats; PCR assay; Pakistan; GENETIC DIVERSITY; GROUND-SQUIRRELS; RODENTS; IDENTIFICATION; INFECTIONS; FLEAS; ECTOPARASITES; PREVALENCE; GENOTYPES; PATTERNS;
D O I
10.1016/j.actatropica.2023.106940
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Bartonella can infect a variety of mammals including humans and has been detected in the Americas, Europe, Africa, and Asia. Roughly two-thirds of identified Bartonella species are found and maintained in rodent res-ervoirs, with some of these species linked to human infections. Rodents (N=236) were caught from the Sahiwal division of Punjab, Pakistan and tested for Bartonella using PCR targeting gltA and rpoB genes, followed by sequencing of rpoB-positive samples. Genetic relatedness to other published Bartonella spp. rpoB gene sequences were examined using BLAST and phylogenetic analysis. Overall, 7.62% (18/236) of rodents were positive for both gltA and rpoB fragments. Rattus rattus and R. norvegicus had 7.94% (12/151) and 7.05% (6/85) positivity rates for Bartonella DNA, respectively. Phylogenetic analysis revealed a close relatedness between Bartonella spp. from Pakistan to Bartonella spp. from China, Nepal, and Malaysia. This study is the first reported detection of Bartonella spp. in R. rattus and R. norvegicus from the Sahiwal area of Punjab, Pakistan.
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页数:8
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