Exosomal miRNA Profiling in Vitreous Humor in Proliferative Diabetic Retinopathy

被引:11
作者
Kot, Agnieszka [1 ]
Kaczmarek, Radoslaw [1 ]
机构
[1] Wroclaw Med Univ, Dept Ophthalmol, PL-50556 Wroclaw, Poland
关键词
miRNA; vitreous; PDR; exosomes; MICRORNA EXPRESSION PROFILES; RATS;
D O I
10.3390/cells12010123
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
MicroRNAs (miRNAs) are small noncoding RNAs which mediate some of the pathological mechanisms of diabetic retinopathy. The aim of this study was to identify differentially expressed miRNAs in the vitreal exosomes of proliferative diabetic retinopathy (PDR) patients and non-diabetic controls. Exosomes were extracted from the vitreous samples of 10 PDR patients and 10 controls. The expression of 372 miRNAs was determined using a quantitative polymerase chain reaction (qPCR) panel. We have demonstrated a significant dysregulation in 26 miRNAs. The most remarkable findings include a profound attenuation of the miR-125 family, as well as enhanced miR-21-5p expression in the diabetic samples. We also showed the downregulation of miR-204-5p and the upregulation of let-7g in PDR compared to the controls. This study identified miR-125 and miR-21 as potential targets for further functional analysis regarding their putative role in the pathogenesis of PDR.
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页数:12
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共 48 条
[1]   High glucose-induced epithelial-mesenchymal transition contributes to the upregulation of fibrogenic factors in retinal pigment epithelial cells [J].
Che, Di ;
Zhou, Ti ;
Lan, Yuqing ;
Xie, Jinye ;
Gong, Haijun ;
Li, Chaoyang ;
Feng, Juan ;
Hong, Honghai ;
Qi, Weiwei ;
Ma, Caiqi ;
Wu, Qiyuan ;
Yang, Xia ;
Gao, Guoquan .
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2016, 38 (06) :1815-1822
[2]   Pathogenic Role of microRNA-21 in Diabetic Retinopathy Through Downregulation of PPARα [J].
Chen, Qian ;
Qiu, Fangfang ;
Zhou, Kelu ;
Matlock, H. Greg ;
Takahashi, Yusuke ;
Rajala, Raju V. S. ;
Yang, Yanhui ;
Moran, Elizabeth ;
Ma, Jian-xing .
DIABETES, 2017, 66 (06) :1671-1682
[3]   Exosomes provide a protective and enriched source of miRNA for biomarker profiling compared to intracellular and cell-free blood [J].
Cheng, Lesley ;
Sharples, Robyn A. ;
Scicluna, Benjamin J. ;
Hill, Andrew F. .
JOURNAL OF EXTRACELLULAR VESICLES, 2014, 3 (01)
[4]   Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis [J].
Doyle, Laura M. ;
Wang, Michael Zhuo .
CELLS, 2019, 8 (07)
[5]   microRNA Expression Profile in the Vitreous of Proliferative Diabetic Retinopathy Patients and Differences from Patients Treated with Anti-VEGF [J].
Friedrich, Julian ;
Steel, David H. ;
Schlingemann, Reinier O. ;
Koss, Michael J. ;
Hammes, Hans-Peter ;
Krenning, Guido ;
Klaassen, Ingeborg .
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY, 2020, 9 (06) :1-10
[6]   Control of glucose homeostasis and insulin sensitivity by the Let-7 family of microRNAs [J].
Frost, Robert J. A. ;
Olson, Eric N. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (52) :21075-21080
[7]   MicroRNA-200b Expression in the Vitreous Humor of Patients with Proliferative Diabetic Retinopathy [J].
Gomaa, Amir Ramadan ;
Elsayed, Eman Tayae ;
Moftah, Reham Fadl .
OPHTHALMIC RESEARCH, 2017, 58 (03) :168-175
[8]   Enhanced ROBO4 is mediated by up-regulation of HIF-1α/SP1 or reduction in miR-125b-5p/miR-146a-5p in diabetic retinopathy [J].
Gong, Qiaoyun ;
Xie, Jia'nan ;
Li, Ying ;
Liu, Yang ;
Su, Guanfang .
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2019, 23 (07) :4723-4737
[9]   Sorting Mechanisms for MicroRNAs into Extracellular Vesicles and Their Associated Diseases [J].
Groot, Michael ;
Lee, Heedoo .
CELLS, 2020, 9 (04)
[10]   The Aflibercept-Induced MicroRNA Profile in the Vitreous of Proliferative Diabetic Retinopathy Patients Detected by Next-Generation Sequencing [J].
Guo, Ju ;
Zhou, Pengyi ;
Liu, Zhenhui ;
Dai, Fangfang ;
Pan, Meng ;
An, Guangqi ;
Han, Jinfeng ;
Du, Liping ;
Jin, Xuemin .
FRONTIERS IN PHARMACOLOGY, 2021, 12