Ultraviolet-visible spectroscopy combined with machine learning as a rapid detection method to the predict adulteration of honey

Honey is often adulterated with inexpensive and artificial sweeteners. To overcome the time-consuming honey adulteration tests, which require precision, chemicals, and sample prepara-tion, it is needful to develop trustworthy analytical methods to assure its authenticity. In the present study, the potential of ultraviolet-visible spectroscopy (UV-Vis) in predicting the sucrose content was evaluated by using Support Vector Regression (SVR) and Partial Least Square Regression (PLSR). To predict the sucrose content based on diagnostic wavelengths, a Point Spectro Transfer Function (PSTF) was evaluated using Multiple Linear Regression (MLR). For this purpose, the spectra of authentic (n = 12), commercial (n = 12), and adulterated (n = 16) honey samples were recorded. Four distinguished wavelengths from correlation analysis between su-crose content and spectra absorption were 216, 280, 316, and 603 nm. The SVR performed better calibration model than the PLSR estimations (RMSE = 0.97, and R2 = 0.98). The predictive models result revealed that both models had high accuracy for the sucrose content estimation. This study proved that UV-Vis spectroscopy provides an economical alternative for the rapid quantification of adulterated honey samples with sucrose.