Study on Extraction, Physicochemical Properties, and Bacterio-Static Activity of Polysaccharides from Phellinus linteus

被引:13
作者
Gao, Nengbin [1 ]
Zhang, Weijia [1 ]
Hu, Dianjie [1 ]
Lin, Guo [1 ]
Wang, Jingxuan [1 ]
Xue, Feng [2 ]
Wang, Qian [1 ]
Zhao, Hongfei [1 ]
Dou, Xin [1 ]
Zhang, Lihong [1 ]
机构
[1] Changchun Univ, Coll Food Sci & Engn, Changchun 130012, Peoples R China
[2] Jilin Prov Changbai Forest Management Bur, Baishan 134499, Peoples R China
来源
MOLECULES | 2023年 / 28卷 / 13期
关键词
Phellinus linteus; ultrasonic extraction; chemical analyses; anti-bacterial effect; ANTIOXIDANT ACTIVITIES; OPTIMIZATION; PHYTOCHEMISTRY; PHARMACOLOGY; MUSHROOMS;
D O I
10.3390/molecules28135102
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We optimized an ultrasound-assisted extraction process of Phellinus linteus mycelium polysaccharides (PLPs) and studied their monosaccharide composition and bacteriostatic properties. Based on a single-factor experiment, a three-factor, three-level Box-Behnken design was used to optimize the ultrasound-assisted extraction process of PLP, using the yield of PLP as the index. The chemical composition and monosaccharide composition of PLP were determined by chemical analysis and HPLC analysis, respectively. Microscopic morphological analysis of the surface of PLP was performed via swept-surface electron microscopy. The bacteriostatic properties of PLP were determined using the spectrophotometric turbidimetric method. The results showed that the best extraction process of PLP with ultrasonic assistance achieved a result of 1:42 g/mL. In this method, the ultrasonic temperature was 60 & DEG;C, ultrasonic extraction was performed for 20 min, and the yield of PLP was 12.98%. The monosaccharide composition of PLP mainly contains glucose (Glc), mannose (Man), galactose (Gal), and glucuronic acid (GlcA). The intracellular polysaccharide of Phellinus igniarius Mycelia (PIP) is an irregular spherical accumulation, the surface is rough and not smooth, and the extracellular polysaccharide (PEP) is a crumbly accumulation. PIP has a stronger inhibitory ability for S. aureus and E. coli and a slightly weaker inhibitory effect for B. subtilis; the inhibitory effect of PEP on S. aureus, E. coli, and B. subtilis is slightly inferior to that of PIP.
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页数:15
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