Towards virtual histology with X-ray grating interferometry

被引：7

作者：

Polikarpov, M. ^{[1
,2
]}

Vila-Comamala, J. ^{[1
]}

Wang, Z. ^{[1
,2
,8
]}

Pereira, A. ^{[1
,2
]}

van Gogh, S. ^{[1
,2
]}

Gasser, C. ^{[2
]}

Jefimovs, K. ^{[1
]}

Romano, L. ^{[1
,2
]}

Varga, Z. ^{[3
]}

Lang, K. ^{[4
,5
]}

Schmeltz, M. ^{[1
]}

Tessarini, S. ^{[1
,2
]}

Rawlik, M. ^{[1
,2
]}

Jermann, E. ^{[6
]}

Lewis, S. ^{[7
]}

Yun, W. ^{[7
]}

Stampanoni, M. ^{[1
,2
]}

机构：

[1] Paul Scherrer Inst, Swiss Light Source, CH-5232 Villigen, Switzerland

[2] Swiss Fed Inst Technol, Inst Biomed Engn, CH-8092 Zurich, Switzerland

[3] Univ Hosp Zurich, Dept Pathol & Mol Pathol, CH-8091 Zurich, Switzerland

[4] Lund Univ, Dept Diagnost Radiol, Translat Med, Lund, Sweden

[5] Skane Univ Hosp, Unilabs Mammog Unit, Malmo, Sweden

[6] Ecole Polytech Fed Lausanne, Lausanne, Switzerland

[7] Sigray Inc, Concord, CA 94520 USA

[8] Tsinghua Univ, Dept Engn Phys, Beijing 100080, Peoples R China

来源：

SCIENTIFIC REPORTS | 2023年 / 13卷 / 01期

基金：

欧洲研究理事会;

关键词：

PHASE-CONTRAST; SYNCHROTRON-RADIATION; MAMMOGRAPHY; VISUALIZATION; RESOLUTION;

D O I：

10.1038/s41598-023-35854-6

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Breast cancer is the most common type of cancer worldwide. Diagnosing breast cancer relies on clinical examination, imaging and biopsy. A core-needle biopsy enables a morphological and biochemical characterization of the cancer and is considered the gold standard for breast cancer diagnosis. A histopathological examination uses high-resolution microscopes with outstanding contrast in the 2D plane, but the spatial resolution in the third, Z-direction, is reduced. In the present paper, we propose two high-resolution table-top systems for phase-contrast X-ray tomography of soft-tissue samples. The first system implements a classical Talbot-Lau interferometer and allows to perform ex-vivo imaging of human breast samples with a voxel size of 5.57 mu m. The second system with a comparable voxel size relies on a Sigray MAAST X-ray source with structured anode. For the first time, we demonstrate the applicability of the latter to perform X-ray imaging of human breast specimens with ductal carcinoma in-situ. We assessed image quality of both setups and compared it to histology. We showed that both setups made it possible to target internal features of breast specimens with better resolution and contrast than previously achieved, demonstrating that grating-based phase-contrast X-ray CT could be a complementary tool for clinical histopathology.

引用

页数：11