High-throughput creation of Nicotiana tabacum gene-targeted mutants based on CRISPR/Cas9

被引：7

作者：

Chen, Yudong ^{[1
]}

Xiang, Haiying ^{[1
]}

Jia, Ling ^{[2
]}

Yang, Qianxu ^{[1
]}

Zhang, Jianduo ^{[1
]}

Jiang, Jiarui ^{[1
]}

Zeng, Wanli ^{[1
]}

Deng, Lele ^{[1
]}

Jin, Jingjing ^{[3
]}

Gao, Qian ^{[1
]}

Li, Xuemei ^{[1
]}

机构：

[1] Yunnan Acad Tobacco Sci, Kunming 650106, Peoples R China

[2] Southwest Univ, Biol Sci Res Ctr, Chongqing 400715, Peoples R China

[3] Zhengzhou Tobacco Res Inst CNTC, China Tobacco Gene Res Ctr, Zhengzhou 450001, Peoples R China

来源：

PLANT CELL REPORTS | 2023年 / 42卷 / 12期

关键词：

D O I：

10.1007/s00299-023-03050-5

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Key message4382 available sgRNAs targeting 1060 tobacco genes were obtained, and 10,682 targeted mutants were created using high-throughput methods. Four optimization experiments were established to solve problems encountered during genetic transformation.

引用

页码：2039 / 2042

页数：4

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PLANT SCIENCE, 2023, 326

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