The Morphology Dependent Interaction between Silver Nanoparticles and Bovine Serum Albumin

被引：6

作者：

Zhang, Jingyi ^{[1
]}

Fu, Xianjun ^{[1
]}

Yan, Changling ^{[2
]}

Wang, Gongke ^{[1
]}

机构：

[1] Henan Normal Univ, Henan Engn Res Ctr Design & Recycle Adv Electroche, Sch Mat Sci & Engn, Xinxiang 453007, Peoples R China

[2] Henan Normal Univ, Collaborat Innovat Ctr Henan Prov Green Mfg Fine C, Sch Chem & Chem Engn, Key Lab Green Chem Media & React,Minist Educ, Xinxiang 453007, Peoples R China

来源：

MATERIALS | 2023年 / 16卷 / 17期

基金：

中国国家自然科学基金;

关键词：

silver nanoparticle; morphology; bovine serum albumin; protein corona; PROTEIN ADSORPTION; GOLD; STABILITY; SURFACE; NANOSPHERES; CHEMISTRY; NANORODS; CITRATE; SIZE;

D O I：

10.3390/ma16175821

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Biological applications of silver nanoparticles (AgNPs) depend on the covalently attached or adsorbed proteins. A series of biological effects of AgNPs within cells are determined by the size, shape, aspect ratio, surface charge, and modifiers. Herein, the morphology dependent interaction between AgNPs and protein was investigated. AgNPs with three different morphologies, such as silver nanospheres, silver nanorods, and silver nanotriangles, were employed to investigate the morphological effect on the interaction with a model protein: bovine serum albumin (BSA). The adsorptive interactions between BSA and the AgNPs were probed by UV-Vis spectroscopy, fluorescence spectroscopy, dynamic light scattering (DLS), Fourier transform infrared spectrometry (FTIR), transmission electron microscopy (TEM), and circular dichroism (CD) techniques. The results revealed that the particle size, shape, and dispersion of the three types of AgNPs markedly influence the interaction with BSA. Silver nanospheres and nanorods were capsulated by protein coronas, which led to slightly enlarged outer size. The silver nanotriangles evolved gradually into nanodisks in the presence of BSA. Fluorescence spectroscopy confirmed the static quenching the fluorescence emission of BSA by the three AgNPs. The FTIR and CD results suggested that the AgNPs with different morphologies had different effects on the secondary structure of BSA. The silver nanospheres and silver nanorods induced more pronounced structural changes than silver nanotriangles. These results suggest that the formation of a protein corona and the aggregation behaviors of AgNPs are markedly determined by their inherent morphologies.

引用

页数：13