Qianjin Wenwu decoction suppresses renal interstitial fibrosis by enhancing the degradation of extracellular matrix in mice with unilateral ureteral obstruction

被引:3
作者
Jin, Chengshan [1 ,4 ]
Wu, Xiaotian [1 ]
You, Yue [1 ]
Wang, Yuing [2 ]
Wu, Jing [2 ]
Zuo, Along [1 ,3 ]
Zheng, Yan [1 ]
Guo, Jianpeng [1 ,3 ]
机构
[1] Yanbian Univ, Coll Pharm, Minist Educ, Key Lab Nat Med Changbai Mt, Yanji 133002, Peoples R China
[2] Yanbian Univ Hosp, Yanji 133002, Peoples R China
[3] Yanbian Univ, Coll Pharm, Yanbian Med & Hlth Ind Pilot Base, Yanji 133002, Peoples R China
[4] Yanbian Chaoyao Biotechnol Co Ltd, Yanji 133002, Peoples R China
关键词
QianJin Wenwu Decoction (QWD); Diabetic kidney disease (DKD); Pharmacodyamic material basis; Molecular docking; TGF-beta; 1; Extracellular matrix (ECM); DIABETIC-NEPHROPATHY; TGF-BETA/SMAD; BETA; PROTECTS;
D O I
10.1016/S1875-5364(23)60434-0
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Diabetic kidney disease (DKD) is the most common complication of diabetes mellitus (DM). Qianjin Wenwu decoction (QWD), a well-known traditional Korean medicine, has been used for the treatment of DKD, with satisfactory therapeutic effects. This study was designed to investigate the active components and mechanisms of action of QWD in the treatment of DKD. The results demonstrated that a total of 13 active components in five types were found in QWD, including flavonoids, flavonoid glycosides, phenylpropionic acids, saponins, coumarins, and lignins. Two key proteins, TGF-ss 1 and TIMP-1, were identified as the target proteins through molecular docking. Furthermore, QWD significantly suppressed Scr and BUN levels which increased after unilateral ureteral obstruction (UUO). Hematoxylin & eosin (H&E) and Masson staining results demonstrated that QWD significantly alleviated renal interstitial fibrosis in UUO mice. We also found that QWD promoted ECM degradation by regulating MMP-9/TIMP-1 homeostasis to improve renal tubulointerstitial fibrosis and interfere with the expression and activity of TGF-ss 1 in DKD treatment.These findings explain the underlying mechanism of QWD for the treatment of DKD, and also provide methodological reference for investigating the mechanism of traditional medicine in the treatment of DKD.
引用
收藏
页码:253 / 262
页数:10
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