Parasympathetic-macrophages-ductal epithelial cells axis promotes female rat submandibular gland regeneration after excretory duct ligation/ deligation

被引：1

作者：

Wang, Bin ^{[1
]}

Li, Dan ^{[1
]}

Li, Jing ^{[1
]}

An, Wei ^{[2
]}

Li, Zhilin ^{[3
]}

Shao, Qi ^{[4
]}

Qin, Lizheng ^{[1
]}

机构：

[1] Capital Med Univ, Beijing Stomatol Hosp, Dept Oral & Maxillofacial & Head & Neck Oncol, Tian Tan Xi Li 4, Beijing 100050, Peoples R China

[2] Shanxi Med Univ, Shanxi Prov Peoples Hosp, Dept Oral & Maxillofacial Surg, Shuang Ta Si St 29, Taiyuan 030012, Peoples R China

[3] Canc Hosp Affiliated Shanxi Med Univ, Shanxi Prov Canc Hosp, Shanxi Hosp Affiliated Canc Hosp, Chinese Acad Med Sci,Dept Head & Neck Oncol, Workers New Village 3, Taiyuan 030013, Peoples R China

[4] Changsha Stomatol Hosp, Dept Oral & Maxillofacial Surg, You Yi Rd 389, Changsha 410004, Peoples R China

来源：

ARCHIVES OF ORAL BIOLOGY | 2023年 / 145卷

基金：

中国国家自然科学基金;

关键词：

Parasympathetic; Macrophages; Interleukin-6; Submandibular gland; Regeneration; SALIVARY; LIVER; PROLIFERATION; ACTIVATION; INNERVATION; PATHWAY; SEX;

D O I：

10.1016/j.archoralbio.2022.105586

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Objective: Submandibular gland (SMG) regeneration depends on parasympathetic signals that modulate this process primarily by promoting ductal epithelial cell proliferation. The purpose of this study was to elucidate the mechanism underlying of parasympathetic regulation on salivary gland regeneration.Materials and methods: We performed duct ligation/deligation with or without chorda lingual innervation in female Sprague-Dawley rats. Clodronate liposomes were administered retroductally and intravenously into the SMG to deplete macrophages. The proliferative ability and cell cycle-related genes of SMG were assessed using western blotting, quantitative real-time PCR, and immunohistochemical staining.Results: Parasympathetic stimulation boosted Interleukin-6 (IL-6) release by macrophage via muscarinic signal-ling. This process was suppressed by denervation, muscarinic blockade, or SMG macrophage depletion. SMG IL-6 release led to signal transducer and activator of transcription 3 (STAT3) phosphorylation in ductal epithelial cells to promote proliferation and regeneration by upregulating the expression of cell cycle-related genes. SMG parasympathetic denervation and macrophage depletion suppressed cell cycle-related genes upregulation and ductal cells proliferation.Conclusions: These data indicated that the parasympathetic-macrophage-ductal epithelial cells axis promote SMG regeneration after duct ligation and deligation.

引用

页数：10

共 54 条

[1] Loss of NF-κB activation in Kupffer cell-depleted mice impairs liver regeneration after partial hepatectomy [J].