Bioinspired porous three-coordinated single-atom Fe nanozyme with oxidase-like activity for tumor visual identification via glutathione

被引：99

作者：

Chen, Da ^{[1
]}

Xia, Zhaoming ^{[2
]}

Guo, Zhixiong ^{[1
]}

Gou, Wangyan ^{[1
]}

Zhao, Junlong ^{[3
]}

Zhou, Xuemei ^{[4
]}

Tan, Xiaohe ^{[1
]}

Li, Wenbin ^{[1
]}

Zhao, Shoujie ^{[3
]}

Tian, Zhimin ^{[1
]}

Qu, Yongquan ^{[1
]}

机构：

[1] Northwestern Polytech Univ, Sch Chem & Chem Engn, Key Lab Special Funct & Smart Polymer Mat, Minist Ind & Informat Technol, Xian 710072, Peoples R China

[2] Southern Univ Sci & Technol, Dept Chem, Shenzhen 518055, Peoples R China

[3] Fourth Mil Med Univ, Dept Med Genet & Dev Biol, State Key Lab Canc Biol, Xian 710032, Peoples R China

[4] Wenzhou Univ, Key Lab Carbon Mat Zhejiang Prov, Wenzhou 325035, Peoples R China

来源：

NATURE COMMUNICATIONS | 2023年 / 14卷 / 01期

基金：

中国国家自然科学基金;

关键词：

OXYGEN REDUCTION; IRON; ELECTROCATALYST; SITES;

D O I：

10.1038/s41467-023-42889-w

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Inspired by structures of natural metalloenzymes, a biomimetic synthetic strategy is developed for scalable synthesis of porous Fe-N-3 single atom nanozymes (pFeSAN) using hemoglobin as Fe-source and template. pFeSAN delivers 3.3- and 8791-fold higher oxidase-like activity than Fe-N-4 and Fe3O4 nanozymes. The high catalytic performance is attributed to (1) the suppressed aggregation of atomically dispersed Fe; (2) facilitated mass transfer and maximized exposure of active sites for the created mesopores by thermal removal of hemoglobin (2 similar to 3 nm); and (3) unique electronic configuration of Fe-N-3 for the oxygen-to-water oxidation pathway (analogy with natural cytochrome c oxidase). The pFeSAN is successfully demonstrated for the rapid colorimetric detection of glutathione with a low limit of detection (2.4 nM) and wide range (50 nM-1 mM), and further developed as a real-time, facile, rapid (similar to 6 min) and precise visualization analysis methodology of tumors via glutathione level, showing its potentials for diagnostic and clinic applications.

引用

页数：13