A fluorescent magnetic nanosensor for imidacloprid based on the incorporation of polymer dots and Fe3O4 nanoparticles into the covalent organic framework

A magnetic nanoprobe was designed for imidacloprid by encapsulating nonconjugated polymer dots (NCPDs) and Fe3O4 nanoparticles in the covalent organic framework (COF). The fluorescence intensity of the COF-based nanocomposite is markedly suppressed by imidacloprid. As the absorption spectrum of imidacloprid was close to the band-gap of the NCPDs, and due to the presence of a nitro group (as an electron acceptor), the electrons can be easily transferred from the conduction band of NCPDs to the LUMO of imidacloprid, so fluorescence quenching was more likely to have been caused by the electron transfer process. The COF-based nanosensor was used for the determination of imidacloprid in the linear range 1.3-130 nM with a detection limit of 1.2 nM. The high sensitivity of the nanoprobe for imidacloprid is due to the combination of COF benefits (accumulation of the imidacloprid into the COF cavities) and the high adsorption ability of the Fe3O4 nanoparticles, which leads to further enrichment of imidacloprid. The magnetic nature of the nanocomposite enables the preconcentration and easy separation of the analyte, and so reduces matrix interference and lowers the detection limits. The practicality of this nanoprobe was confirmed by quantification of imidacloprid in the wastewater and fruit juice samples.